刺五加-云芝乙醇提取物对小鼠抗抑郁作用

目的:研究刺五加-云芝不同比例混合后醇提物对小鼠的抗抑郁作用，并筛选出具有最强抗抑郁活性的比例，并探讨其抗抑郁作用机理。方法:通过75%乙醇超声辅助提取有效成分，小鼠给药剂量为4.55 mg/kg，连续给药14 d；测定刺五加-云芝（1:1、1:2、1:3、1:4、1:5、5:1、4:1、3:1、2:1）不同比例醇提取物对小鼠行为绝望模型的影响。通过腹膜内注射利血平建立小鼠抑郁模型；ELISA法检测小鼠海马中单胺类神经递质5-HT、NE以及小鼠血清中促炎和抗炎细胞因子IL-1β、IL-10的含量和水平变化。结果:刺五加-云芝（1:2、1:1）组和单一实验组均能不同程度降低小鼠在行为绝望模型中的失望时间（P<0.05），抑制由利血平引起的体温下降及眼睑下垂（P<0.05），1:2组能显著提高小鼠脑中5-HT、NE及小鼠血清中IL-10的含量（P<0.01，P<0.05），下调血清中IL-1β的含量（P<0.01）。刺五加-云芝1:2配比组作用效果最好。结论:刺五加-云芝配伍组合对小鼠抑郁模型有一定的作用，作用机制可能与增加抑郁模型小鼠脑中5-HT、NE及小鼠血清中IL-10的含量，降低小鼠血清中IL-1β的含量有关。

Effects of Acanthopanax senticosus-Coriolus versicolor Ethanolic Extract on Anti-depressive in Mice

Objective: To study the anti-depressant effects of Acanthopanax senticosus and Coriolus versicolor ethanolic extracts in different proportions in mice, to screen out the proportion with the strongest anti-depressant activity, and explore its mechanism of action. Methods: The effective components were extracted by 75% ethanol with the ultrasound-assisted extraction method, and the mice were given the drug continuously for 14 days and the dose was 4.55 mg/kg. The effects of different proportions of Acanthopanax senticosus and Coriolus versicolor (1:1, 1:2, 1:3, 1:4, 1:5, 5:1, 4:1, 3:1, 2:1) ethanolic extracts on the behavior of mice were determined. Through intraperitoneal injection of reserpine, themice model of depression was established to detect the content of monoamine neurotransmitters in the brain of the mice and on serum IL-10 and IL-1β content in mice, so as to determine the effects of different proportion of Acanthopanax senticosus -Coriolus versicolor ethanolic extract on the depression effect of reserpine. Results: Different proportions of Acanthopanax senticosus and Coriolus versicolor (1:2, 1:1) ethanolic extracts markedly reduced immobility time in the tail suspension test (TST) and forced swimming test (FST) compared with the single drug group of mice (P<0.05), and they can inhibit the symptom of the hypothermia and ptosis caused by reserpine (P<0.05). Moreover, the research also found that the cotent of 5-hydroxytry ptamine (5-HT), norepinephrine (NE) were significantly increased (P < 0.01, P < 0.05) and IL-10 were significantly increased (P<0.01) in 1:2 groups. The best proportions of Acanthopanax senticosus and Coriolus versicolor ethanolic extracts was 1:2 (P<0.05). Conclusions: Acanthopanax senticosus and Coriolus versicolor ethanolic extracts possessed antidepressant effects in mice, which might be partially related to up-regulate IL-10, increase 5-HT, NE levels in the hippocampus and down-regulate IL-1β.