多汁乳菇多糖对小鼠急性酒精性肝损伤的保护作用

目的:以多汁乳菇精多糖（Refined polysaccharide from Lactarius volemus Fr，RPLV）为试验材料，探究RPLV对小鼠急性酒精性肝损伤的保护作用。方法:55只小鼠随机分为空白对照组、模型组、阳性对照组、RPLV高、低剂量组（400、100 mg/kg·BW），连续给药30 d后，断食12 h，空白组以外的其余各组按照12 mL/kg的剂量灌胃浓度为50%乙醇溶液，建立急性酒精性损伤模型。12 h后取血，采用全血细胞分析仪测定外周白细胞（White blood cell，WBC）、红细胞（Red blood cell，RBC）和血小板（Blood platelet，PLT）含量，同时取肝脏，按照试剂盒测定血液及肝脏中的丙二醛（malondialdehyde，MAD）、还原型谷胱甘肽（glutathione，GSH）、过氧化氢酶（catalase，CAT）、超氧化物歧化酶（superoxide dismutase，SOD）、谷胱甘肽过氧化物酶（glutathione peroxidase，GSH-Px）、甘油三酯（triglyceride，TG）、谷草转氨酶（aspartate amino transferase，AST）和谷丙转氨酶（aspartate transaminase，ALT）水平，并观察小鼠的肝脏病理切片及超微结构。结果:模型组小鼠肝脏及血液中的各项生理生化指标与空白对照组差异显著（P<0.01），RPLV高、低剂量组肝脏和血液中的SOD、CAT、GSH-Px活力和GSH含量显著上升（P<0.01或P<0.05），RPLV高剂量组ALT、AST活力及MDA、TG含量显著下降（P<0.01或P<0.05）；病理组织切片和超微结构的观察显示，受试样品RPLV可明显改善肝脏受损情况。结论:多汁乳菇精多糖可以一定程度上改善酒精对肝脏的损伤。

Hepatoprotective Effect of Refined Plysaccharide from Lactarius volemus Fr on Alcohol-induced Acute Liver Injury in Mice

Objective: To explore the hepatoprotective effect of refined polysaccharide from Lactarius volemus Fr on alcohol-induced acute liver injury in mice. Methods: Fifty-five mice were randomly divided into groups as follows: Normal control group, model group, positive control group, RPLV high and low dose group(400、100 mg/kg·BW). Continuous administration for 30 days, the mice in each group were deprived of food for 12 h. Except the normal control group, the other groups were given 50% ethanol by gavage at a dose of 12 mL/kg to establish an acute alcoholic injury model. At the end of the experiment, blood was collected, and then the mice were sacrificed with cervical dislocation to harvest their liver tissues. Red blood cell(RBC), white blood cell(WBC) and blood platelet(PLT) were measured using a blood cell counter. Superoxide dismutase(SOD), glutathione peroxidase(GSH-Px), catalase(CAT), aspartate amino transferase(AST), aspartate transaminase(ALT) activity and triglyceride(TG), malondialdehyde(MAD), glutathione(GSH) content in liver and serum were measured. Histopathological liver and ultrastructural changes were examined. Results: Each physiological and biochemical index in liver and serum signifificantly differed(P<0.01) between the normal control and model groups. Compared with the model group, the RPLV dose groups in liver and serum showed significant or extremely significant decrease in MDA, TG content and ALT、AST activity(P<0.05 or P<0.01), while the SOD, GSH-Px, CAT activity and GSH content in high dose group increased significantly or extremely significantly(P<0.05 or P<0.01). And by histological examination of pathological sections and ultrastructure, each test samples romoted recovery from hepatic tissue injury. Conclusion: Refined polysaccharide from Lactarius volemus Fr had a signifificant hepatoprotective effect on alcohol-induced acute liver injury in mice.