沙棘多糖抗运动性疲劳及抗氧化作用的研究

研究沙棘多糖（polysaccharide from Hippophae rhamnoides，PBP）对长时间过量运动小鼠体力疲劳的缓解作用。小鼠分为空白组、PBP 低（100 mg/kg）、中（200 mg/kg）、高（400 mg/kg）剂量组，连续灌胃15 d，记录力竭游泳时间，测定血乳酸（lactic acid，LA）、血清尿素氮（blood urea nitrogen，BUN）、肝脏中肝糖原（hepaticr glycogen，HG）含量，以及体外 DPPH 自由基、ABTS+自由基清除率和总还原能力。结果表明，与空白对照组相比，PBP能够显著延长小鼠负重游泳时间（P<0.05），其中中剂量组游泳时间增加极显著（P<0.01）。PBP中、高剂量组极显著（P<0.01）的降低了血清中BUN、LA含量，中剂量组极显著（P<0.01）的增加了肝脏中HG的储存量。体外抗氧化结果表明，沙棘多糖对 DPPH 自由基、ABTS+自由基都具有一定的清除能力，其 IC₅₀值分别为0.81、2 mg/mL，当浓度为1 mg/mL时，总还原能力为0.727。结果表明沙棘多糖具有一定的缓解体力疲劳、增强小鼠运动能力和抗氧化的作用。

Anti Exercise Fatigue and Antioxidant of Polysaccharide from Hippophae rhamnoides

To study the effect of polysaccharide from Hippophae rhamnoides (PBP) on mitigating physical fatigue in mice with prolonged excessive exercise. The mice were divided into blank group, low (100 mg/kg), middle (200 mg/kg) and high (400 mg/kg) dose group of PBP, and fed continuous gavage for 15 d. Exhaustive swimming time was recorded. Then, the levels of blood lactic acid (LA), blood urea nitrogen (BUN) and the contents of glycogen. The results showed that, compared with the blank group, PBP significantly prolonged exhaustive swimming time (P<0.05). Exhaustive swimming time in middle PBP group increased significantly (P<0.01). And it significantly decreased the levels of BUN and LA in middle and high PBP groups. The level of HG in middle PBP group increased significantly (P<0.01). The antioxidant activity experiment in vitro showed that, the polysaccharideof Hippophae rhamnoides had certain scavenging ability to DPPH and ABTS+ free radical. And the level of IC₅₀ was 0.81 and 2 mg/mL. When the concentration of polysaccharide of Hippophae rhamnoides was 1 mg/mL, the total reducing power was 0.727. Therefore, the polysaccharide of Hippophae rhamnoides can relieve physical fatigue, enhance the exercise ability of mice and have effect on antioxidant.