| 耐胃肠道环境芽孢杆菌菌株的筛选 |
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| 引用本文： | 闵钟熳,牛天贵,岳喜庆.耐胃肠道环境芽孢杆菌菌株的筛选[J].食品工业科技,2007(5):113-115. |
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| 作者姓名： | 闵钟熳 牛天贵 岳喜庆 |
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| 作者单位： | 1. 沈阳农业大学食品学院,辽宁沈阳,110161
2. 中国农业大学食品科学与营养工程学院,北京,100083 |
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| 摘 要： | 以来源于健康动物肠道的芽孢杆菌为出发菌株,通过模拟体内高胆盐环境(胆盐含量0.3%),来监测菌株的耐受能力;测定在人工胃液、肠液环境中作用不同时间后的存活菌数的方法,来初步筛选能够作为益生菌的芽孢杆菌。实验结果表明,有3株芽孢杆菌分别为B5329、B22、B544,在胆酸盐、人工胃液、人工肠液中表现出了很强的耐受能力。
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| 关 键 词： | 芽孢杆菌 胆酸盐 人工胃液 人工肠液 |
| 文章编号： | 1002-0306(2007)05-0113-03 |
| 修稿时间： | 2006年12月6日 |
Isolation of Bacillus strains with tolerance to gastrointestinal environment |
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| Min Zhongman et al.Isolation of Bacillus strains with tolerance to gastrointestinal environment[J].Science and Technology of Food Industry,2007(5):113-115. |
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| Authors: | Min Zhongman |
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| Affiliation: | Min Zhongman et al |
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| Abstract: | Bacillus strains were screened from intestines of healthy animals. Using bile salt medium, the tolerance of Bacillus was studied in the simulated natural (bile salt content 0.3%) environment. Then the survival rates of bacteria after incubation in artificial gastric juice and intestinal environment for different time were detected. Those having strong tolerance were preliminarily regarded as probiotics. The experimental results show that there were three Bacillus strains: B5329, B22 and B544 in bile salt, artificial gastric and intestinal juice that demonstrated strong tolerance. |
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| Keywords: | Bacillus bile salts artificial gastric juice artificial intestinal fluid |
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