具有潜在降血糖作用乳酸菌的筛选

以商业菌株鼠李糖乳杆菌LGG为对照,从实验室保藏的乳酸菌中筛选出具有良好α-葡萄糖苷酶抑制率和胃肠道耐受能力的菌株。结果表明,36株乳酸菌中存在4株具有较高α-葡萄糖苷酶抑制率的植物乳杆菌YDA11、OD1、OD2和YDB24,其抑制率分别为28.37%、30.26%、30.26%和33.18%,且抑制率均与对照菌株LGG无显著性差异(p>0.05),同时,菌株OD1和OD2对α-淀粉酶的抑制率分别为30.84%和35.17%,显著高于对照菌株LGG(p <0.05)。在耐酸实验中,在pH2.5的环境中胁迫8 h后,菌株YDB24与菌株OD2耐酸能力较好,存活率分别为96.01%与96.83%;在耐胆盐实验中,菌株OD2的耐受能力最强,在0.3%胆盐环境中胁迫后存活率为94.81%,且菌株OD2在胃液和小肠液中存活率分别为94.22%和79.99%。植物乳杆菌OD2为具有良好胃肠道耐受能力的潜在降血糖菌株。

Screening of Lactic Acid Bacteria with Potential Hypoglycemic Effect

Compared with the commercial strain Lactobacillus rhamnosus LGG,strains with good α-glucosidase inhibition rate and gastrointestinal resistance were screened from laboratory-preserved lactic acid bacteria.The results showed that,there were4 strains of Lactobacillus plantarum YDA11,OD1,OD2 and YDB24 with higher α-glucosidase inhibition rates among 36 strains of lactic acid bacteria,and their inhibition rates were 28.37%,30.26%,30.26% and 33.18% separately.These inhibition rates were not significantly different with LGG strain( p > 0.05).In addition,the(-amylase inhibition rates of strains OD1 and OD2 were 30.84% and 35.17%,which were significantly higher than LGG( p < 0.05).In the acid-tolerant experiment,strain YDB24 and strain OD2 showed better acid resistance.After 8 h of acidic environment at pH2.5,their survival rates were 96.01% and96.83%.In the bile salt test,the comprehensive tolerance of strain OD2 was the strongest,whose survival rate after stress in0.3% bile salt MRS broth was 94.81%.The survival rates of strain OD2 in simulated succus gastricus and succus entericus were 94.22% and 79.99%,respectively.In conclusion,Lactobacillus plantarum OD2 was screened as a potential hypoglycemic strain with good gastrointestinal resistance.