不同品种甜荞芽中酚类成分的比较

本文建立了甜荞芽5种酚类成分的测定方法,并且对7种甜荞芽中酚类成分进行比较分析。采用超高效液相色谱(UPLC)法检测甜荞中5种多酚成分含量,色谱条件为ACQUITYUPLC? BEHC₁₈色谱柱(2.1 mm×50 mm,1.7 μm),流动相甲醇(A)-0.20%冰乙酸水溶液(B),检测波长为254 nm,流速为0.20 mL/min,进样量为1 μL;并采用高效液相色谱法对甜荞芽中的矢车菊素-3-O-葡萄糖苷和矢车菊素-3-O-芸香糖苷成分的含量进行检测,用DPS 14.50软件对甜荞芽第7、10 d酚类成分的含量进行分析。结果表明,建立的超高效液相色谱法准确可靠,甜荞芽第7、10 d酚类成分总含量具有显著性差异(P<0.05),其中第7、10 d荭草素、异荭草素、牡荆素、异牡荆素、芦丁总含量最高的品种分别是赤甜1号、品甜2号,分别为43.66、40.82 mg/g,含量最低的品种分别是宁荞1号、定甜3号,分别为20.45、14.13 mg/g;矢车菊素-3-O-葡萄糖苷和矢车菊素-3-O-芸香糖苷总含量最高的分别是红花甜荞、宁甜1号,分别为218.47、282.95 mg/kg,最低的分别是赤甜1号、丰甜1号,分别为49.91、88.35 mg/kg。本实验为甜荞芽质量评价提供了依据,为后续研究奠定了基础。

Comparison of Phenolic Compounds in Different Varieties of Buckwheat Sprout

A method for the determination of five phenolic components in buckwheat sprout was established,and the phenolic components in 7 kinds of buckwheat sprout were compared and analyzed. Five polyphenols in buckwheat were determined by ultra-high performance liquid chromatography(UPLC)system equipped with a ACQUITYUPLC? BEHC₁₈(2.1 mm×50 mm,1.7 μm)chromatographic column,with the mobile phase of methanol-0.20% glacial acetic acid solution at a flow rate of 0.20 mL/min.The column temperature and the detection wavelength set at 35℃ and 254 nm respectively. High performance liquid chromatography(HPLC)was used to determine the contents of cypermethrin-3-O-glucoside and cypermethrin-3-O-rutin in buckwheat sprout. The content of phenols in buckwheat sprout was determined by DPS 14.50 software on the 7th and 10th day. The results showed that the HPLC method was accurate and reliable,and the total contents of phenols in buckwheat sprout on the 7th and 10th day were significantly different(P<0.05). On the 7th and 10th days,the highest total contents of orientin,isoorientin,vitexin,isovitexin and rutin were Chitian 1,Pingtian 2,which were 43.66,40.82 mg/g,respectively,and the lowest varieties were Ningqiao 1 and Dingtian 3,which were 20.45,14.13 mg/g,respectively. On the 7th and 10th day,the highest total contents of cornulin-3-O-glucoside and cornarin-3-O-rutin were Honghua,Ningtian 1,which were 218.47,282.95 mg/kg,respectively,and the lowest were Chitian 1,Fengtian 1,which were 49.91,88.35 mg/kg,respectively. This experiment provides the basis for the quality evaluation of buckwheat sprout and lays a foundation for further study.