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| 响应曲面法优化猕猴桃酒酵母培养条件 | |
| 引用本文： | 汤高奇,陈月英,岳田利,柳艳霞.响应曲面法优化猕猴桃酒酵母培养条件[J].食品工业科技,2010(4). |
| 作者姓名： | 汤高奇 陈月英 岳田利 柳艳霞 |
| 作者单位： | 1. 河南农业职业学院,河南郑州,451450;西北农林科技大学,陕西杨凌,712100 2. 河南农业职业学院,河南郑州,451450 3. 西北农林科技大学,陕西杨凌,712100 4. 河南农业大学,河南郑州,450002 |
| 基金项目： | 国家西部专项(2001BA901A19) |
| 摘 要： | 利用JMP和Box-Behnken响应曲面设计(RSM),研究了培养条件对猕猴桃酒酵母WF25增殖作用的影响。研究结果表明:温度、装液量、转速、初始pH和培养时间对猕猴桃酒酵母WF25细胞的增殖均有极显著的影响。当培养条件为温度28.5℃、初始pH4.5、转速175r/min、装液量65mL和时间30h时,生物量的预测值可达3.06352×108个/mL。 |
| 关 键 词： | 猕猴桃酒酵母 培养条件 响应曲面 |
Optimization of cultivated conditions of kiwifruit wine yeast by response surface methodology |
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| TANG Gao-qi,CHEN Yue-ying,YUE Tian-li,LIU Yan-xia.Optimization of cultivated conditions of kiwifruit wine yeast by response surface methodology[J].Science and Technology of Food Industry,2010(4). | |
| Authors: | TANG Gao-qi CHEN Yue-ying YUE Tian-li LIU Yan-xia |
| Affiliation: | TANG Gao-qi1,2,CHEN Yue-ying1,YUE Tian-li2,LIU Yan-xia3(1.Henan Vocational College of Agriculture,Zhengzhou 451450,China,2.Northwest Sci-Tech University of Agriculture& Forestry,Yangling 712100,3.Henan Agriculture University,Zhengzhou 450002,China) |
| Abstract: | The effects of cultivated conditions on WF_(25) proliferation role of kiwifruit wine yeast were studied.The result of JMP and Box- Behnken response surface methodology (RSM) indicated: temperature, volume of medium, rotated speed,initial pH and time had very significant influence.Optimum cultivated conditions of cell proliferation were temperature 28.5℃, initial pH4.5, rotated speed 175r/min, volume of medium 65mL and time 30h, the prediction value of biomass reach to 3.06352 × 10~8/mL. |
| Keywords: | kiwifruit wine yeast cultivated conditions response surface methodology(RSM) |
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