| 鸭肝丁酰胆碱酯酶的纯化与酶学性质研究 |
| |
| 引用本文： | 朱鸿,李想韵,邓玉,王松,付伟丽,唐靓婷,诰赵伟,唐云明.鸭肝丁酰胆碱酯酶的纯化与酶学性质研究[J].食品工业科技,2011(1):95-99. |
| |
| 作者姓名： | 朱鸿 李想韵 邓玉 王松 付伟丽 唐靓婷 诰赵伟 唐云明 |
| |
| 作者单位： | 西南大学生命科学学院,三峡库区生态环境教育部重点实验室,重庆市甘薯工程研究中心,重庆,400715 |
| |
| 基金项目： | 重庆市科委资助项目(CSCT,2004AC1012) |
| |
| 摘 要： | 目的:获得鸭肝丁酰胆碱酯酶纯品并对其酶学性质进行研究。方法:采用丙酮脱脂、酸沉淀、硫酸铵分级沉淀、DEAE-Sepharose阴离子交换层析和Sephacryl S-200凝胶层析方法,分离纯化鸭肝丁酰胆碱酯酶;采用SDS-聚丙烯酰胺凝胶电泳法进行纯度鉴定和酶相对分子量测定。结果:从鸭肝中分离纯化获得电泳纯的丁酰胆碱酯酶,纯化倍数为156.45倍,酶活回收率为23.60%,比活达17.21U/mg。酶相对分子量为388.85kDa,亚基相对分子量为64.70kDa。推测该酶由六个相同亚基构成。该酶最大紫外吸收为278nm。酶催化碘化硫代丁酰胆碱水解的最适pH为8.0,最适温度为35℃。该酶在pH3.0～10.0区域较稳定;在40℃以下处理1h,酶活力保持稳定。Zn2+、Mn2+和Cu2+对该酶具有显著的抑制作用。以碘化丁酰硫代胆碱为底物,测定该酶的表观Km为71.15μmol/L,没有过量底物抑制现象。结论:成功分离纯化获得丁酰胆碱酯酶,该酶具有较好的酸碱耐受性。
|
| 关 键 词： | 鸭肝 丁酰胆碱酯酶 分离纯化 性质 |
Purification and Characterization of the butyrylcholinesterase from duck liver |
| |
| ZHU Hong,LI Xiang-yun,DENG Yu,WANG song,FU Wei-li,TANG Liang-ting,GAO Zhao-wei,TANG Yun-ming.Purification and Characterization of the butyrylcholinesterase from duck liver[J].Science and Technology of Food Industry,2011(1):95-99. |
| |
| Authors: | ZHU Hong LI Xiang-yun DENG Yu WANG song FU Wei-li TANG Liang-ting GAO Zhao-wei TANG Yun-ming |
| |
| Affiliation: | ZHU Hong,LI Xiang-yun,DENG Yu,WANG Song,FU Wei-li,TANG Liang-ting,GAO Zhao-wei,TANG Yun-ming(School of Life Science,Southwest University,Key Laboratory of Eco-environments in Three GorgesReservoir Region Ministry of Education,Chongqing Sweet Potato Engineering Research Center,Chongqing 400715,China) |
| |
| Abstract: | Objective:To obtain butyrylcholinesterase from duck liver and study on the characterization of the purified product. Methods:The butyrylcholinesterase was extracted by acetone treatment,acid precipitation,ammonium sulfate precipitation,and ion-exchange chromatography on DEAE-Sepharose and gel filtration on Sephacryl S-200.SDS-PAGE was used to identify the purity and relative molecular of the butyrylcholinesterase. Results:The enzyme was purified to electrophoretic homogeneity. It was purified 156.45-fold an... |
| |
| Keywords: | duck liver butyrylcholinesterase purification characterization |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
