| 不同来源异戊烯基焦磷酸异构酶(IDI)和脱氧木酮糖磷酸合成酶(DXS)对重组大肠杆菌番茄红素产量的影响 |
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| 引用本文： | 杨帆,苏卜利,姚青,李华平,朱红惠.不同来源异戊烯基焦磷酸异构酶(IDI)和脱氧木酮糖磷酸合成酶(DXS)对重组大肠杆菌番茄红素产量的影响[J].食品工业科技,2018,39(18):131-136. |
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| 作者姓名： | 杨帆 苏卜利 姚青 李华平 朱红惠 |
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| 作者单位： | 1. 广东省微生物研究所, 广东省菌种保藏与应用重点实验室, 广东省微生物应用新技术公共实验室, 省部共建华南应用微生物国家重点实验室, 广东广州 510070;2. 华南农业大学农学院, 广东广州 510642;3. 华南农业大学园艺学院, 广东广州 510642 |
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| 基金项目： | 南粤微生物优秀人才培育基金(GDIMYET20160204)广东省自然科学基金-博士启动纵向协同(2017A03030513)广东省科学院实施创新驱动发展能力建设专项资金项目(2017GDASCX-0821,2017GDASCX-0402)广东省科学院科研平台环境与能力建设专项资金项目(2016GDASPT-0302)。广东省科技创新领军人才项目(2015TX01N036) |
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| 摘 要： | 为获得更多异戊烯基焦磷酸异构酶(isopentenyl diphosphate isomerase,IDI)和脱氧木酮糖磷酸合成酶(1-deoxyxylulose-5-phosphate synthase,DXS)相关功能基因资源,本研究选取来源于大肠杆菌(Escherichia coli)、枯草芽孢杆菌(Bacillus subtilis)、粘细菌(Myxococcus stipitatus)、戈壁奇球菌(Deinococcus gobiensis)的4种IDI和DXS,分别克隆到表达载体,将其与含有合成番茄红素基因的pTrc99aEBI质粒共同在大肠杆菌中进行异源表达。结果表明,来源于粘细菌的IDI和DXS可以获得最高的番茄红素产量,分别达到了10.86、7.94 mg/g DCW。协同表达经过密码子优化的IDI、DXS,番茄红素产量达到了15.26 mg/g DCW。本研究通过比较不同来源的IDI和DXS,获得了新的基因资源。
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| 关 键 词： | 番茄红素 异戊烯基焦磷酸异构酶(IDI) 脱氧木酮糖磷酸合成酶(DXS) 大肠杆菌 代谢工程 |
| 收稿时间： | 2018-01-18 |
Effects of Different Isopentenyl Diphosphate Isomerase (IDI) and 1-Deoxyxylulose-5-phosphate Synthase (DXS) on Lycopene Production in Engineering Escherichia coli Strains |
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| YANG Fan,SU Bo-li,YAO Qing,LI Hua-ping,ZHU Hong-hui.Effects of Different Isopentenyl Diphosphate Isomerase (IDI) and 1-Deoxyxylulose-5-phosphate Synthase (DXS) on Lycopene Production in Engineering Escherichia coli Strains[J].Science and Technology of Food Industry,2018,39(18):131-136. |
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| Authors: | YANG Fan SU Bo-li YAO Qing LI Hua-ping ZHU Hong-hui |
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| Affiliation: | 1. Guangdong Institute of Microbiology, State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangzhou 510070, China;2. College of Agricultural, South China Agricultural University, Guangzhou 510642, China;3. College of Horticulture, South China Agricultural University, Guangzhou 510642, China |
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| Abstract: | In order to obtain more relevant functional gene resources of isopentenyl diphosphate isomerase (IDI) and 1-deoxyxylulose-5-phosphate synthase (DXS), four IDI and DXS genes were cloned from different species, including Escherichia coli, Bacillus subtilis, Myxococcus stipitatus and Deinococcus gobiensis were heterologous expressed in Escherichia coli with pTrc99aEBI. Results showed that, MyxoIDI and MyxoDXS were the most efficient species for lycopene production and the yield of lycopene reached to 10.86, 7.94 mg/g DCW. The yield of lycopene reached 15.26 mg/g DCW by co-expression of the codon optimized IDI、DXS. This study described new genetic resources for lycopene production by comparing the different IDI and DXS. |
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