扛板归多糖含量测定方法的建立

建立扛板归多糖的含量测定方法,效应面分析法研究扛板归多糖含量测定的最佳条件。以葡萄糖为标准品,采用苯酚-硫酸法和3,5-二硝基水杨酸法,分别测定扛板归水提液中总糖和还原糖的含量,从而计算多糖含量;在单因素试验的基础上,运用三因素五水平的星点设计-效应面分析法优选多糖测定的最佳条件。最佳测定条件如下,总糖:苯酚用量1.7 mL,硫酸用量8.7 mL,显色时间11 min;还原糖:显色剂用量2.7 mL,显色时间10 min,显色温度81 ℃。在最佳测定条件下,总糖和还原糖的样品加标回收率分别为102.26%(RSD=4.03%,n=9)和102.89%(RSD=2.66%,n=9)。扛板归多糖含量为46.72 mg/g。本方法灵敏、稳定、重复性好,可用于扛板归多糖含量的测定。

Establish a Determination Method of Polysaccharides from Polygonum perfoliatum L.

To establish a determination method of polysaccharides in Polygonum perfoliatum L. and the optimum polysaccharides determination conditions were investigated using the response surface methodology (RSM). The contents of total sugars and reducing sugars were measured by phenol-sulfuric acid method and 3, 5-dinitrosalicylic acid (DNS) method, respectively. These test used glucose as the standard substance. Thus the polysaccharides contents was calculated. On the basic of the results of the single factor experiments, based on a five level, three variable central composite design (CCD) -RSM, was employed to obtain the best possible combination for polysaccharides determination. The optimum determination conditions were as follows:total sugars:phenol reagent (1.7 mL), sulfuric acid (8.7 mL), reaction time (11 min);reducing sugars:DNS reagent (2.7 mL), coloration time (10 min), coloration temperature (81℃). Under these onditions, average recoveries of total sugars and reducing sugars in the test were 102.26% with RSD=4.03% (n=9) and 102.89% with RSD=2.66% (n=9), respectively. The content of polysaccharides in Polygonum perfoliatum L. was 46.72 mg/g. This method is sensitive and stable, which could be used to determine the contents of polysaccharides in Polygonum perfoliatum L. with good reproducibility.