风味酵母A10-2的高密度培养及其生长动力学

为实现一株自分离风味酵母的高密度培养及生长动力学预测模型构建，本文对从酱油发酵醪中筛选的产4-乙基愈创木酚的风味酵母A10-2进行了高密度培养工艺的研究，对培养基种类（氮、碳源）、浓度进行了研究及优化，并对酵母的生长、基质消耗过程进行了数学模型的构建和验证。结果表明:磷酸二氢铵作为无机氮源，浓度0.2 g/100 mL为最适氮源流加补料方案；使用糖蜜作为唯一碳源时，培养液中持续流加保持总糖浓度为0.4~0.6 g/100 mL可获得最佳菌体生长速度；A10-2酵母的生长符合Logistic方程S形曲线，基质（总糖）消耗符合Leudeking-Priet方程，比生长速率最大值μ_m为0.4764 h?1，最大菌体生长得率系数Y_G值为0.5879 g/g，维持系数m_s=0.0127 g·L?1·h?1。所建立模型能够较好的描述酵母在高密度培养过程中的生长和耗糖情况，具有预测指导意义。

High Density Culture Process and Growth Kinetics of Flavor Yeast A10-2

In order to realize the high-density culture and establish the predictive growth kinetics model of a self-isolated flavor yeast, the high-density culture process was studied of 4-ethyl-2-methoxyphenol producing yeast A10-2, which screened from soy sauce fermentation mash. The type of culture medium (nitrogen and carbon source) and concentration were studied and optimized. The growth kinetics and the substrate (total sugar) consumption models were established and verified. The results showed that (NH₄) H₂PO₄ fed to make the concentration of culture medium 0.2 g/100 mL was the best inorganic source of nitrogen. To obtain the best cell growth rate, molasses as the only source of carbon, should be fed which controlled the concentration of total sugar in the culture medium to 0.4~0.6 g/100 mL. The growth of A10-2 yeast followed the S-shaped curve of a Logistic model, and the substrate (total sugar) consumption followed the Leudeking-Priet equation. The maximum obtained biomass specific growth rate μ_m was 0.4764 h?1, while the maximum biomass growth yield coefficient Y_G was 0.5879 g/g. The maintenance coefficient was 0.0127 g·L?1·h?1. The established models could better describe the growth and sugar consumption of yeast in the process of high-density culture, and have predictive significance.