酶法制备透明质酸寡糖及其透皮吸收活性研究

为研究透明质酸寡糖的透皮吸收活性，采用透明质酸裂解酶对透明质酸进行酶法降解，制备透明质酸寡糖，通过红外光谱与质谱对透明质酸寡糖进行分析，并对其吸湿性能、体表保湿性能和透皮吸收性能进行评价。结果显示，透明质酸裂解酶酶解制备透明质酸寡糖的最适底物浓度为1%，最适加酶量为3.0 U/mg，酶解制备透明质酸寡糖基本没有基团脱落，酶解产物以不饱和透明质酸二糖为主。酶法制备的透明质酸寡糖具有优良的体表保湿与透皮吸收性能，在生物化工领域具有广阔的应用前景。

Preparation of Hyaluronic Acid Oligosaccharides by Enzymatic Method and Its Transdermal Absorption Activity

To investigate the transdermal absorption activity of hyaluronic acid oligosaccharides, hyaluronic acid oligosaccharides were prepared by enzymatic degradation of hyaluronic acid using hyaluronic acid lyase, and the hyaluronic acid oligosaccharides were analyzed by infrared spectroscopy and mass spectrometry, and their hygroscopic properties, body surface moisturizing properties and transdermal absorption properties were evaluated. The results showed that the optimum substrate concentration for the enzymatic preparation of hyaluronan oligosaccharides by hyaluronan lysis enzyme was 1%, and the optimum enzyme addition was 3.0 U/mg. The enzymatic preparation of hyaluronan oligosaccharides was free of moieties, and the enzymatic products were mainly unsaturated hyaluronan disaccharides. The enzymatically prepared hyaluronan oligosaccharide had excellent surface moisturizing and transdermal absorption properties, and would have a broad application prospect in the field of biochemicals.