| 固相萃取-高效液相色谱法检测葡萄中氯吡脲的研究 |
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| 引用本文： | 侯玉茹,杨媛,石磊,王宝刚,冯晓元,李喜宏.固相萃取-高效液相色谱法检测葡萄中氯吡脲的研究[J].食品科技,2011(1):255-258. |
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| 作者姓名： | 侯玉茹 杨媛 石磊 王宝刚 冯晓元 李喜宏 |
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| 作者单位： | 天津科技大学食品工程与生物技术学院;北京市农林科学院林业果树研究所;北京农产品质量检测与农田环境监测技术研究中心; |
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| 基金项目： | 北京市科委课题(Z09090501040901) |
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| 摘 要： | 利用固相萃取-高效液相色谱(SPE-HPLC)分离测定葡萄中氯吡脲的残留量。样品匀浆经乙腈提取,固相萃取柱净化后,在流动相为甲醇-纯水(体积比为63:37)、紫外检测波长为260nm的色谱条件下进行分离检测。实验结果表明:经筛选碱性氧化铝柱对样品的净化效果较好,氯吡脲在0.01～1.00μg/mL范围内线性关系良好,相关系数r=0.9999,最低检出浓度为0.0001mg/kg。试样在0.004、0.010、0.050mg/kg3个添加水平下的平均回收率在94.38%～99.33%之间,相对标准偏差(RSD)在1.67%~3.62%之间。利用此方法对市售4个品种葡萄中氯吡脲的残留量进行检测,最终残留均符合标准。
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| 关 键 词： | 氯吡脲 葡萄 固相萃取 高效液相色谱 |
Study of determination of forchlorfenuron in grape by solid-phase extraction and high-performance liquid chromatography |
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| HOU Yu-ru,YANG Yuan,SHI Lei,WANG Bao-gang,FENG Xiao-yuan,LI Xi-hong.Study of determination of forchlorfenuron in grape by solid-phase extraction and high-performance liquid chromatography[J].Food Science and Technology,2011(1):255-258. |
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| Authors: | HOU Yu-ru YANG Yuan SHI Lei WANG Bao-gang FENG Xiao-yuan LI Xi-hong |
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| Affiliation: | HOU Yu-ru1,2,YANG Yuan2,SHI Lei2,WANG Bao-gang2,FENG Xiao-yuan2,3,LI Xi-hong1 (1.Food Engineering and Biotechnology Institute,Tianjin University of Science and Technology,Tianjin 300457,2.Institute of Forestry and Pomology,Beijing Academy of Agriculture and Forestry Science,Beijing 100093,3.Beijing Research Center for Agrifood Testing and Farmland Monitoring,Beijing 100097) |
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| Abstract: | The forchlorfenuron residues in grapes were determined by solid-phase extraction and high- performance liquid chromatography(SPE-HPLC) in this paper.The sample was extracted with acetonitrile,and cleaned up with solid-phase extraction column.Forchlorfenuron was determined by high-performance liquid chromatography at 260 nm using methanol-water(63:37,v/v)as mobile phase.The results indicated that the Al-N column was more effective than others,and the calibration curve showed good linearity when the amount of... |
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| Keywords: | forchlorfenuron grape solid-phase extraction high-performance liquid chromatography(HPLC) |
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