桑叶多糖的化学组成及其对胰脂肪酶的抑制作用研究

将复合酶辅助热水提取所得的桑叶粗多糖依次进行脱蛋白和脱色处理后得到桑叶多糖,研究其化学组成和对胰脂肪酶的抑制作用及作用类型。结果表明,桑叶多糖的总糖含量为58.28%,其单糖组成及摩尔比为甘露糖:鼠李糖:葡萄糖:半乳糖:木糖:阿拉伯糖:葡萄糖醛酸:半乳糖醛酸=8.61:23.42:4.23:12.73:1.31:3.06:41.40:123.32。桑叶多糖对胰脂肪酶有一定抑制作用,其半数抑制浓度(IC50)为10.32 mg/mL,抑制作用类型为非竞争性抑制。桑叶多糖有望作为胰脂肪酶抑制剂用于减肥保健食品的加工。

Chemical Composition of Mulberry Leaf Polysaccharide and its Inhibitory Effect on Pancreatic Lipase

A crude polysaccharide extracted from mulberry leaves with hot water followed by a complex enzyme treatement were deproteinized and decolorized,resulting in a mulberry leaf polysaccharide,and its basic chemical composition was analyzed.Moreover,its inhibition activity on pancreatic lipase was investigated.The results showed that the polysaccharide contained 58.28% of total sugar and composed of mannose,rhamnose,glucose,galactose,xylose,arabinose,glucuronic acid,galacturonic acid in the molar ratio of 8.61:23.42:4.23:12.73:1.31:3.06:41.40:123.32.Mulberry leaf polysaccharide demonstrated a certain inhibitory effect on pancreatic lipase,and its half inhibitory concentration (IC50) was 10.32 mg/mL.It inhibited the enzyme in a non-competitive way.Mulberry leaf polysaccharide can be used as a pancreatic lipase inhibitor in the processing of functional food product for weight loss.