葡萄籽超微粉中多酚成分的检测

对葡萄籽超微粉中的多酚成分进行了分析和测定。儿茶素是葡萄籽多酚中单体的主要成分,又是构成原花青素的主要单体,福林肖卡法测定多酚时采用儿茶素作为对照物来表征葡萄籽多酚含量更准确。经过改进的HPLC方法对没食子酸、儿茶素、表儿茶素三种单体的分离效果较好,具有很好的准确度和精密度,适合于葡萄籽超微粉中的单体检测。经测定,葡萄籽超微粉中,多酚含量10.9%(以儿茶素计),三种单体的总量为1.2%,以总多酚含量减去三种单体总量代表原花青素含量来计算,原花青素含量为9.7%。

Determination of the components of polyphenols in the ultrafine powder of grape seed

The components of polyphenols in the ultrafine powder of grape seeds were measured and analyzed.Catechin is not only the main component of monomeric phenols in the grape seed polyphenols,but also the important material constituting proanthocyanidin.So it is more accurate that the content of polyphenols is expressed as catechin equivalents in the Folin-Ciocalteu method.Gallic acid,catechin and epicatechin of three monomeric phenols was separated well,the improved HPLC method was full of accuracy and precision,suitable for the determination of the three monomeric phenols in the ultrafine powder of grape seeds.The results showed that the contents of polyphenols expressed as catechin equivalents and the three monomeric phenols in the ultrafine powder of grape seed were 10.9% and 1.21%,respectively.The content of proanthocyanidin was 9.7% represented by the difference between amount of polyphenols and the three monomeric phenols.