| 茶多糖酶法提取的优化条件及其对葡萄糖激酶活性的影响 |
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| 引用本文： | 刘育玲,姚开,贾冬英,郑悦.茶多糖酶法提取的优化条件及其对葡萄糖激酶活性的影响[J].食品科技,2010(2). |
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| 作者姓名： | 刘育玲 姚开 贾冬英 郑悦 |
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| 作者单位： | 四川大学轻纺与食品学院; |
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| 基金项目： | 国家自然科学基金项目(20472058) |
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| 摘 要： | 为揭示茶多糖的高提取效率与其高活性之间的相关性,在系统考察胰酶作用条件和冰乙醇用量对其提取效率影响的基础上,采用正交实验对茶多糖的酶法提取条件进行了优化。结果表明,酶解条件和冰乙醇用量对茶多糖的提取效率有较大的影响,获得茶多糖高提取效率(54.70%)所需条件与获得高降糖活性茶多糖(葡萄糖激酶相对酶活比值达3.97)所需条件不同。
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| 关 键 词： | 茶多糖 胰酶 提取条件 提取效率 葡萄糖激酶活性 |
Optimization condition for enzymatic extraction of tea polysaccharide and its effect on glucokinase activity |
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| LIU Yu-ling,YAO Kai,JIA Dong-ying,ZHENG Yue.Optimization condition for enzymatic extraction of tea polysaccharide and its effect on glucokinase activity[J].Food Science and Technology,2010(2). |
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| Authors: | LIU Yu-ling YAO Kai JIA Dong-ying ZHENG Yue |
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| Affiliation: | LIU Yu-ling,YAO Kai,JIA Dong-ying,ZHENG Yue (College of Light Industry , Food,Sichuan University,Chengdu 610065) |
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| Abstract: | In order to investigate the correlation of high-extraction efficiency of tea polysaccharide and its high activity, its enzymatic extraction conditions were optimized by orthogonal experiment based on a systematical study, which is on the effects of pancreatin hydrolysis conditions and ice-ethanol addition on the extraction efficiency of tea polysaccharide. The results showed that enzymatic hydrolysis conditions and ice-ethanol addition exerted a great influence on the extraction efficiency of tea polysaccha... |
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| Keywords: | tea polysaccharide pancreatin extraction conditions extraction efficiency glucokinase activity |
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