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利用钛胶高效液相色谱法测定食品中叶酸含量
引用本文:谢东磊,李荣,谭津,姜子涛.利用钛胶高效液相色谱法测定食品中叶酸含量[J].食品科技,2012(1):250-253.
作者姓名:谢东磊  李荣  谭津  姜子涛
作者单位:天津商业大学生物技术与食品科学学院天津市食品生物技术重点实验室
基金项目:国家自然科学基金项目(20875069)
摘    要:建立了利用钛胶高效液相色谱法测定食品中叶酸含量的方法。色谱条件:钛胶固定相(250mm×4.6mmi.d.,5μm),流动相为水:甲醇=70:30(v/v)、流动相中含磷酸盐(PBS)20mmol/L,流速0.9mL/min,柱温60℃,检测波长280nm。线性范围为5~25μg/mL,检出限1.9220μg/mL,回收率为92.7%~95.1%,RSD(n=8)<0.68%。应用于食品样品中叶酸的测定,结果满意。

关 键 词:叶酸  钛胶固定相  高效液相色谱法

Determination of folic acid in food by titania-based high performance liquid chromatography
XIE Dong-lei,LI Rong,TAN Jin,JIANG Zi-tao.Determination of folic acid in food by titania-based high performance liquid chromatography[J].Food Science and Technology,2012(1):250-253.
Authors:XIE Dong-lei  LI Rong  TAN Jin  JIANG Zi-tao
Affiliation:(Tianjin Key Laboratory of Food Biotechnology,College of Biotechnology and Food Science,Tianjin University of Commerce,Tianjin 300134)
Abstract:A method for the determination of folic acid in food by high performance liquid chromatography on titania has been reported.The pretreated samples were separated with a titania-based column(250×4.6 mm,5 μm).The chromatographic separation conditions were as follows:a mixture of water and methanol in the ratio of 70 to 30 containing 20 m mol/L phosphate used as mobile phase,flow rate of 0.9 mL/min,column temperature 60 ℃,and the detection wavelength 280 nm.The linear range of the method was 5~25 μg/mL with a detection limit of 1.92 μg/mL.The recoveries were between 92.7% and 95.1%.RSD(n=8) was less than 0.68%.The method is applied to determine folic acid in food with satisfactory results.
Keywords:folic acid  titania-based stationary phase  high performance liquid chromatography
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