猪血红蛋白酶解制备ACE抑制肽的研究

本实验选用碱性蛋白酶、胰蛋白酶、胃蛋白酶、风味蛋白酶、中性蛋白酶和木瓜蛋白酶等六种商业蛋白酶在各自最适反应条件下分别水解猪血红蛋白12h,研究其水解产物对血管紧张素转换酶抑制率和蛋白水解度的影响。结果显示:采用胃蛋白酶酶解获得的产物ACE抑制率最高。胃蛋白酶的酶解条件为底物5%(质量分数),酶与底物浓度比E:S=3%,温度37℃,pH2.0,水解4h后其ACE抑制率为81.10%,水解度为6.64%。

Preparation of Angiotensin-I Converting Enzyme Inhibition Peptides from Porcine Hemoglobin

This experiment adopted six kinds of commercial protease Alcalase 2.4L, trypsin, pepsin, flavourzyme, nuetrase AS1398 and papain to hydrolyze porcine hemoglobin in optimum reaction conditions for 12 hours respectively, and the hydrolysates were assayed for the inhibitory activity of angiotensin-I converting enzyme(ACE) and the protein hydrolysis degree.The results showed that the ACE inhibitory activity of pepsin-derived hydrolysates was highest in all the proteases.The condition of enzymatic hydrolysis of pepsin was:substrate concentration was 5%, enzyme/substrate ratio was 3%, temperature was 37℃, pH 2.0, and in the 4th hour the inhibitory activity of ACE reached 81.10% and the degree of hydrolysis was 6.64%.