金银花提取物对冷却肉中假单胞菌黏附性的影响

研究冷却肉中假单胞菌的黏附能力及金银花提取物的降黏附效果。结果表明：从15 份市售冷却肉中分离 得到46 株假单胞菌，经16S rDNA结合VITEK 2系统鉴定为恶臭假单胞菌22 株，占47.8%；荧光假单胞菌10 株，占 21.7%；铜绿假单胞菌6 株，占13.0%；浅黄假单胞菌8 株，占17.4%；33 株（71.7%）假单胞菌具有黏附能力，具有 强黏附能力的菌株占17.4%，其中恶臭假单胞菌数量最多；金银花提取物能够降低4 种假单胞菌的黏附能力，且对 恶臭假单胞菌5-3和铜绿假单胞菌5-1作用效果更强，作用效果与金银花提取物质量浓度有关；金银花提取物质量浓 度高于最小抑菌质量浓度（minimum inhibitory concentration，MIC）时，主要通过抑制菌体生长、减少胞外多糖产 生而降低假单胞菌的黏附能力；金银花提取物质量浓度低于MIC时，主要通过溶解菌体胞外多糖、增强通透性、降 低均匀度而降低假单胞菌的黏附能力。

Effect of Flos Lonicerae Japonicae Extract on the Adhesion Ability of Pseudomonas Strains from Chilled Pork

The adhesion ability of Pseudomonas strains from chilled pork and the inhibitory effect of Flos Lonicerae Japonicae extract on it was analyzed in this study. A total of 46 strains of Pseudomonas strains were isolated from 15 commercial chilled pork samples, including 22 strains of Pseudomonas putida (47.8%), 10 strains of Pseudomonas fluorescens (21.7%), 6 strains of Pseudomonas aeruginosa (13.0%), and 8 strains of Pseudomonas luteola (17.4%), as identified by 16S rDNA sequence analysis and VITEK 2 system. In total 33 (71.7%) of them were found to have adhesion ability, 17.4% of which had strong adhesion ability, mostly Pseudomonas putida. Flos Lonicerae Japonicae extract could reduce the adhesion ability of the four Pseudomonas species, and had stronger effect on P. putida 5-3 and P. aeruginosa 5-1 in a concentration-dependent manner. At concentrations higher than the minimum inhibitory concentration (MIC), it excerted its effect mainly by inhibiting the growth of bacteria and reducing the production of extracellular polysaccharides. At concentrations lower than the MIC, its excerted its effect mainly by dissolving extracellular polysaccharides, consequently increasing the membrane permeability and reducing uniformity.