鸭肉冒充牛羊肉的分子生物学检测

针对送检牛肉和羊肉样品,设计合成检测动物源性成分的通用引物,并对PCR产物测序后在NCBI数据库中进行比对;根据牛、羊、鸭源性成分检测的相关标准,合成引物和探针,进行送检样品中牛、羊和鸭源性成分的实时荧光PCR检测。结果表明:在7份送检样品中4份检测到鸭源性成分,2份检测到牛源性成分,1份检测到羊源性成分。实时荧光PCR方法灵敏性更高、特异性更强、结果判定更为迅速。

Molecular Biological Detection of Adulteration of Mutton and Beef with Duck

A molecular biological method was established to detect mutton and beef samples suspected of being adulterated with duck. Universal primers for the detection of components of animal origin were designed. The PCR amplification products were sequenced before NCBI database searching for sequence alignment. Primers and probes were synthesized according to relevant detection standards for the real-time PCR detection of bovine-origin, sheep-origin and duck-origin components. Of 7 tested samples, 4 contained duck-origin components, 2 contained bovine-origin components, and 1 contained sheep-origin components. This real-time PCR assay was sensitive, specific and rapid.