| 纤维素酶活力测定方法 |
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| 引用本文： | 张瑞萍.纤维素酶活力测定方法[J].印染,2002,28(8):38-39. |
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| 作者姓名： | 张瑞萍 |
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| 作者单位： | 南通工学院,226007 |
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| 摘 要： | 用DNS为显色剂，分别以滤纸和CMC为底物，以滤纸糖酶活性（FPA）和羧甲基纤维素酶活性（CMCase)表征纤维素酶活力，确定酶活测定用波长为530nm，参比溶液应为失活酶，底物和DNS等共热的反应物；比较了两种底物的酶活力测定方法，结果表明，CMCase比FPA高，说明酶对水溶性底物有较高的活力，也表现吸附对酶的活性部位与纤维素分子链段的结合及催化均有很大影响，对于不同牌号的纤维素酶，织物的酶减量率与CMC酶活力关系密切。
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| 关 键 词： | 纤维素酶活力 测定方法 织物 酶减量率 |
| 修稿时间： | 2002年3月15日 |
Activity Determination of Cellulase |
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| Zhang Ruiping.Activity Determination of Cellulase[J].Dyeing and Finishing,2002,28(8):38-39. |
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| Authors: | Zhang Ruiping |
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| Affiliation: | Zhang Ruiping |
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| Abstract: | Using DNA as developer, filter paper and CMC as base, the activity of cellulase was determined and compared by FPA and CMCase, under the condition of wavelength 530nm, and using deactivation enzyme, base, DNS as reference solution. The result showed that the activity of cellulase of CMCase was higher than that of FPA. This means that cellulase has higher activity to soluble base. As for different cellulase with different trademark, there is close relationship between deweighting rate of cellulase and activity of CMC. |
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| Keywords: | Testing Cellulase Activity |
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