蒸汽爆破对甘蔗叶酶法制备低聚木糖的影响

研究了蒸汽爆破对甘蔗叶酶法制备低聚木糖的影响,分别考察了直接干爆、酸浸预处理和碱浸预处理的汽爆效果。得出的结论如下:蒸汽爆破可以有效破坏物料的表面形状,增加与酶接触的表面积,添加酸和碱有利于总糖的溶出,酸性汽爆产生的还原糖比较多,酶解产物中主要是以单糖为主。碱性汽爆的酶解率更高,酶解产物中主要是低聚木糖,占总产物78%~85%,其中木二糖的含量约占40%~50%。碱性汽爆更有利于低聚木糖的制备。

The Influence of Steam Explosion on Enzymatic Preparation of Xylooligosaccharides from Sugarcane Leaves

In this paper,steam explosion in various conditions was studied in order to reveal its impact on preparation of xylooligosaccharides from sugarcane leaves by enzymatic hydrolysis.The results demonstrated that steam explosion could effectively break down the surface of materials and make it easier for enzymetic hydrolysis.The addition of acid or alkli facilitated xylan's release.On one hand,acidic steam-explosion contributed to sugar reduction and was responsible for the poor proportion of xylooligosaccharides in the end-product.On the other hand,alkalic steam-explosion made xylan extraction easy in the form of large molecule and generating less reducing sugar.Higher rate of enzymatic hydrolysis was also attributed to alkalic steam-explosion.Finally,when the steam explosive substance was hydrolyzed by xylanase,it produced 78%~85% xylooligosaccharides including 40%~50% xylobinose.Steam-explosion assisted by alkali was a good method for manufacturing of xylooligosaccharides.