| 纸层析-分光光度法测定发酵液中L-丝氨酸和甘氨酸的含量 |
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| 引用本文： | 卢发,张伟国.纸层析-分光光度法测定发酵液中L-丝氨酸和甘氨酸的含量[J].食品与发酵工业,2005,31(3):119-121. |
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| 作者姓名： | 卢发 张伟国 |
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| 作者单位： | 江南大学工业生物技术教育部重点实验室,无锡,214036 |
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| 摘 要： | 应用“纸层析 分光光度法”对发酵液中的L 丝氨酸和甘氨酸进行了定性分析和定量测定,确立了L 丝氨酸和甘氨酸定量测定条件。研究结果表明,该法对测定L 丝氨酸和甘氨酸含量具有较高的准确度和精密度。而且该法操作简单、易于掌握,非常适合在L 丝氨酸产生菌筛选中应用。
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| 关 键 词： | L-丝氨酸 甘氨酸 纸层析法 |
| 修稿时间： | 2004年9月20日 |
Determination of L-Serine and Glycine in Broth with Paper Chromatography and Spectrophotometer |
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| Lu Fa,Zhang Weiguo.Determination of L-Serine and Glycine in Broth with Paper Chromatography and Spectrophotometer[J].Food and Fermentation Industries,2005,31(3):119-121. |
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| Authors: | Lu Fa Zhang Weiguo |
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| Abstract: | The concentrations of L-serine and glycine in broth were qualitatively and quantitatively determined with paper chromatography and spectrophotometer. The determination conditions of L-serine and glycine were established. It showed that the method is highly accurate and precise in the determination of L-serine and glycine. The method was easy to operate and suitable for the screening of strains producing L-serine. |
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| Keywords: | L-serine glycine paper chromatography |
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