以木质素为载体固态发酵制备生物肥料的工艺条件

以木质素为载体进行了固氮菌CN 2的固体发酵制备生物肥料的实验。结果表明 ,在草浆木质素中加入适量的玉米秸秆粉作为疏松剂 ,可作为固体发酵的良好载体 ,其优点在于可提高空障率 ,有利于散热和传氧。木质素载体在固体发酵中还具有稳定 pH的作用 ,适当提高其含量有利于促进微生物的生长。以固氮菌固体发酵制备木质素生物肥料的最佳工艺条件是 :工业木质素与玉米秸秆粉的质量比是 1∶0 75 ,接种量为 10 % ,培养温度 3 5℃ ,固液质量比为 1∶2 ,料层厚度为7 5cm。此培养条件下固氮菌活菌数最高可达 4 7× 10 10 /g。

Study on Technological Conditions of SSF to Prepare the Bio-fertilizer with Lignin as Carrier

Experiments were performed to prepare the bio fertilizer by solid state fermentation(SSF)of Azotobacter CN 2 with lignin as carrier The results showed that the pulp straw lignin,with suitable amount of corn stalk powder as loose substrate,is good carrier for SSF to prepare bio fertilizer,because it can increase the interstice ratio of substrate and further help to disseminate heat and transfer oxygen Suitable amount of lignin has the effect on stabilizing pH in SSF and promotes the growth of microorganism The optimal technological conditions of preparing lignin bio fertilizer are as follow When ratio of pulp straw lignin to corn stalk is 1∶0 75,inoculation amount is 10%,culture temperature is 35℃,ratio of solid carrier to liquid medium is 1∶2 and substrate thickness is 7 5cm,the number of viable bacteria can reach to 4 7×10 10 /g