安卡红曲霉液态发酵产色素及糖化酶的研究

对1株红曲霉(Monascus anka sp.)液态发酵产色素及糖化酶的培养条件进行了优化。研究了碳源、氮源、装液量、接种量等因素对色素积累及糖化酶活力的影响。并通过正交试验确定了最佳培养条件:甘油10%,蛋白胨1.5%,温度32℃,初始pH5.75,接种量6%,装液量75mL/500mL,种龄5d。验证实验表明,以色价最佳组合条件发酵时,发酵液色价达到263.5U/mL;以糖化酶活最佳组合条件发酵时,酶活达到409.6U/mL;以色价和糖化酶活力最佳组合条件发酵时,发酵液色价为260.3U/mL,糖化酶活为404.0U/mL。

Study on Submerged Fermentation Conditions of a Strain Monascus anka sp. Producing Pigment and Glucoamylase

The parameters of producing red pigment and glucoamylase in a strain Monascus anka sp.were optimized under submerged incubation.The effects of carbon sources,nitrogen sources,loading amount and inoculating amount on the accumulation of pigment and glucoamylase were investigated.The optimum parameters obtained by orthogonal test were as follows:glycerol 10%,peptone 1.5%,temperature 32℃,initial pH value 5.75,inoculating amount 6%,loading amount 75 mL/500 mL conical flask and inoculum age 5 d.The results of validating tests indicated that the color value produced by this Monascus anka sp.reached 263.5 U/mL under optimum pigment accumulation condition,the glucoamylase activity reached 409.6 U/mL under optimum glucoamylase accumulation condition,and color value and glucoamylase activity reached 260.3 U/mL and 404.0 U/mL,respectively under optimum pigment and glucoamylase accumulation condition.