山药多糖RDPS-I组分的纯化及理化性质的研究

山药经水浸提分离 ,浸提液脱蛋白 ,透析 ,乙醇沉淀物经DEAE -5 2纤维素及SephadexG -10 0色谱纯化得白色粉末状多糖RDPS -I。SepharoseCL -6B凝胶色谱分析表明RDPS -I为多糖纯品。定性化学反应表明RDPS -I不含核酸、蛋白质、酚类物质和糖醛酸 ,是一种非淀粉类中性纯粹多糖 ,比旋光度 α] 2 2D(H2 O)为 +188 4(c =0 8) ,特性粘度 η]为 16 48× 10 -3 (mL/g) ,相对分子质量为 42 2 0 0 ,完全酸水解后纸层析及气相色谱分析确定RDPS -I的糖基组成为葡萄糖、甘露糖和半乳糖 ,摩尔比为 1∶0 37∶0 11。

Purification and Physico-chemical Properties Analysis of Chinese Yam Polysaccharides Friction RDPS-I

A polysaccharide named RDPS-I was isolated from Chinese yam with water extraction, alcohol sedimentation, Sevag deprotein, DEAE-52 column and Sephadex G-100 column, its purity was identified by Sepharose CL-6B. RDPS-I was a non-starch polysaccharide, did not contain any protein, nuclear acid, polyphenol and uronic acid, its specific rotary was +188.4(H 2O, c=0.8), specific viscosity was 16.48×10 -3 mL/g, average molecular weight was 42 200u. The composed monosaccharide were identified by GC and paper chromography as glucose,mannose and galactose with a molar ratio of 1∶0.37∶0.11.