| Trichoderma viride菌生物量测定及其纤维素酶合成特征 |
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| 引用本文： | 吴克,杨本宏,张洁,刘斌,蔡敬民,潘仁瑞,Marinus Meiners.Trichoderma viride菌生物量测定及其纤维素酶合成特征[J].食品与发酵工业,2002,28(8):9-12. |
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| 作者姓名： | 吴克 杨本宏 张洁 刘斌 蔡敬民 潘仁瑞 Marinus Meiners |
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| 作者单位： | 1. 合肥联合大学生物工程教研室,合肥,230022
2. University of Applied Sciences Oldenburg/Ostfriesland/Wilhelmshaven, Emden, Germany, 26723 |
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| 基金项目： | 德国下萨克森州科文部资助项目，安徽省教育厅自然科学基金资助项目 (No .2 0 0 0j12 6 7zc) |
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| 摘 要： | 利用HPLC法测定Trichodermaviride菌固态发酵曲中的麦角固醇含量。研究了麦角固醇与菌丝体间的关系。该菌固态曲中麦角固醇分离条件以 1∶2 5 (m/v)的丙酮抽提 1 5h为最佳。当固态发酵培养至 69h时 ,曲中的生物量达到最大值 ,为每克干曲中含有 0 5 75 g菌丝体。此时该菌所产生CMC酶和FP酶活力均达到最大值 ,呈现正相关性 ,说明这 2种酶的合成特征均为同步合成型 ,而C1 酶活力高峰滞后 ,出现在 72h。
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| 关 键 词： | 固态发酵 麦角固醇 Trichodermaviride 生物量 纤维素酶 |
| 修稿时间： | 2002年4月22日 |
Trichoderma viride Biomass Assay and Its Cellulase Synthesis Characteristic |
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| Marinus Meiners.Trichoderma viride Biomass Assay and Its Cellulase Synthesis Characteristic[J].Food and Fermentation Industries,2002,28(8):9-12. |
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| Authors: | Marinus Meiners |
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| Abstract: | The biomass of Trichoderma viride in solid state fermentation was assayed by the HPLC The relationship of fungi to the ergosterol content was studied The investigation results showed that the best conditions for extracting ergosterol from mycelium in the culture were using acetone with the ratio of 1∶25 (m/v) for 1 5?h The biomass in the culture reached to maximum 0 575?g mycelium/g dry medium after cultured 69h together with CMCase and Fpase activities The relationship of biomass to CMCase and to FPase showed positive relevance This indicated that these two enzymes were belong to synchronic synthesis model C 1ase synthesis speed was delayed to that of CMCase and FPase, its maximum activity appeared after cultured 72?h |
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| Keywords: | solid state fermentation ergosterol Trichoderma viride biomass cellulase |
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