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高效液相色谱法测定菠萝中多酚类化合物
引用本文:易湘茜,韦保耀,滕建文,高程海.高效液相色谱法测定菠萝中多酚类化合物[J].食品与发酵工业,2006,32(2):99-101.
作者姓名:易湘茜  韦保耀  滕建文  高程海
作者单位:广西大学轻工与食品工程学院,南宁,530004
摘    要:利用高效液相色谱法(HPLC)分析了多酚类物质在菠萝果皮、果肉、果心中的含量及分布情况。色谱条件:色谱柱为 Diamonsil C_(18)柱(250 mm×4.6 mm,5μm),流动相为乙腈和0.3%醋酸溶液,柱温为25℃,流速为 1.0mL/min,线性梯度洗脱,检测波长为280nm。在此色谱条件下,各组分均得到良好分离。经测定,菠萝果皮中含有4种酚类物质,为没食子酸、儿茶素、表儿茶素、阿魏酸,含量较高;果肉及果心中酚类物质为表儿茶素和阿魏酸,含量较低。

关 键 词:菠萝  多酚  高效液相色谱
收稿时间:08 22 2005 12:00AM
修稿时间:2005-08-222006-01-04

Determination of the Phenolic Compounds in Pineapple by High Performance Liquid Chromatography
Yi Xiangxi,Wei Baoyao,Teng Jianwen,Gao Chenghai.Determination of the Phenolic Compounds in Pineapple by High Performance Liquid Chromatography[J].Food and Fermentation Industries,2006,32(2):99-101.
Authors:Yi Xiangxi  Wei Baoyao  Teng Jianwen  Gao Chenghai
Abstract:The content of phenolic compounds in pineapple peel,core and pulp were studied by high perfor- mance liquid chromatography(HPLC) on a C18column(250 mm×4.6 mm,5μm).The mobile phase was acetoni- trile with 0.3% acetic acid solution,The compositions were separated completely under the condition of:flow rate 1.0 mL/min,column temperature 25℃,280 nm wavelength of detector.The determination results show that there were four hydrone phenolic compounds in pineapple peel,galic acid,catechin,epicatechin,and felinic acid; while in pineapple pulp and core,only little epicatechin and felinic acid were detected.
Keywords:pineapple  phenolic compounds  high performance liquid chromatography
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