高效液相色谱法测定动物源性食品中角黄素、虾青素的研究

研究并建立了高效液相色谱测定动物源性食品中角黄素、虾青素的方法。采用加有抗氧剂BHT的乙腈提取、正己烷脱脂制备样液。黄鱼、鳗鱼、鸡肉、鸡蛋、鸭肝、猪肾、牛奶等样品添加0.1～1 mg/kg的角黄素和虾青素时,角黄素和虾青素平均回收率范围分别为84.2%～103.1%和83.1%～98.7%,RSD范围分别为3.0%～10.5%和2.0%～8.9%,测定低限0.1 mg/kg。

Determination of Canthaxanthin and Astaxanthin in Animal Origin Food by HPLC

A method had been developed to determine canthaxanthin and astaxanthin in animal origin food by high performance liquid chromatography.The canthaxanthin and astaxanthin in the test sample are ex- tracted with acetonitrile containing BHT and defatted with hexane.In the levels of 0.1～1 mg/kg spike con- centration in large yellow croaker muscle,eel muscle,chicken muscle,chicken egg,duck liver,pig kidney and milk,The spiike average recoveries of canthaxanthin and astaxanthin are in the range of 84.2-103.1% and 83.1-98.7%,respectively,and the relative standard deviation (RSD)falls in the range of 3.0-10.5% and 2.0-8.9%,respectively.The limits of detection of canthaxanthin and astaxanthin are all 0.1 mg/kg.