| 枣花蜜蛋白质的电泳行为及其荧光光谱分析 |
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| 引用本文： | 邓建军,杨海霞,程妮,王毕妮,高慧,曹炜.枣花蜜蛋白质的电泳行为及其荧光光谱分析[J].食品与发酵工业,2012,38(10):155-158. |
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| 作者姓名： | 邓建军 杨海霞 程妮 王毕妮 高慧 曹炜 |
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| 作者单位： | 1. 西北大学食品科学与工程系,陕西西安,710069
2. 西安交通大学医学院公共卫生系营养与食品卫生学学科,陕西西安,710061 |
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| 基金项目： | 国家自然科学基金，陕西省农业科技创新项目，陕西省自然科学基金 |
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| 摘 要： | 以枣花蜜为试材,分析了不同产地的枣花蜜蛋白质的电泳行为及其荧光光谱特征。结果表明:不同地区枣花蜜的蛋白质含量存在明显差异,在供试样品中陕西佳县蛋白质含量最高(1 468.18μg/g),而富平蛋白质含量最低(719.03μg/g);SDS-PAGE分析发现,陕西省不同地区枣花蜜蛋白质主要电泳谱带差异不大,但不同省份的枣花蜜蛋白质电泳谱带差异较大,表现出特有的蛋白质谱带;荧光光谱分析发现,不同地区枣花蜜蛋白质的最大荧光吸收峰和强度存在明显差异,说明枣花蜜蛋白质组成和结构存在一定的差异。
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| 关 键 词： | 枣花蜜 蛋白质 电泳 荧光光谱 |
SDS-PAGE Electrophoresis and Fluorescence Spectroscopy to Analysis Chinese Date Honey Protein |
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| Deng Jian-jun,Yang Hai-xia,Cheng Ni,Wang Bi-ni,GAO Hui,Cao Wei.SDS-PAGE Electrophoresis and Fluorescence Spectroscopy to Analysis Chinese Date Honey Protein[J].Food and Fermentation Industries,2012,38(10):155-158. |
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| Authors: | Deng Jian-jun Yang Hai-xia Cheng Ni Wang Bi-ni GAO Hui Cao Wei |
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| Affiliation: | 1(School of Chemical Engineering,Northwest University,Xi’an 710069,China) 2(College of Medical,Xi’an Jiaotong University,Xi’an 710061,China) |
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| Abstract: | In this paper,SDS-PAGE electrophoresis and fluorescence spectroscopy were used to analysis Chinese date honey(CDH) protein.It is shown that there were significant differences on protein content of CDH from different areas.For example,the protein content of CDH in Jiaxian was the highest(1468.18 μg/g),whereas that in Fuping was the lowest(719.03 μg/g).The main protein bands of CDH in different areas in Shaanxi were little difference.However,the protein bands of CDH from different province showed highly significant difference,with specific protein bands.Meanwhile,the maximum peak and intensity of CDH protein fluorescence from difference areas have significant differences,indicating significant differences on protein composition and structure of CDH. |
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| Keywords: | chinese data honey protein electrophoresis fluorescence spectroscopy |
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