| 应用响应面法优化虫草发酵核苷类物质 |
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| 引用本文： | 汤蕾,余晓斌,郝学财,胡卫珍.应用响应面法优化虫草发酵核苷类物质[J].食品与发酵工业,2005,31(11):60-62. |
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| 作者姓名： | 汤蕾 余晓斌 郝学财 胡卫珍 |
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| 作者单位： | 江南大学生物工程学院,工业生物技术教育部重点实验室,无锡,214036 |
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| 摘 要： | 采用响应面法(RSM)对冬虫夏草茵(Cordycepssinensis)CS-wx液态发酵生产核苷类物质的3种主要培养基成分——麦芽糖、葡萄糖和蛋白胨进行优化，采用多元二次回归方程拟合3种因素与核苷类物质含量为响应值之间的函数关系。经回归分析表明，上述3种因素对核苷类物质的含量有显著影响。通过岭脊分析获得对核苷类物质含量有显著影响的因素最佳浓度为：麦芽糖2.17％，葡萄糖2.95％，蛋白胨2.74％，通过HPLC法检测腺苷含量为1.02mg／g，较原始培养基腺苷含量0.85mg／g提高了20％。
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| 关 键 词： | 冬虫夏草 核苷类 腺苷 响应面分析 岭脊分析 HPLC |
| 收稿时间： | 06 3 2005 12:00AM |
| 修稿时间： | 09 9 2005 12:00AM |
Optimizing Fermentation Nucleotides of Cordyceps sinensis by Response Surface Method |
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| Tang Lei,Yu Xiaobin,Hao Xuecai,Hu Weizhen.Optimizing Fermentation Nucleotides of Cordyceps sinensis by Response Surface Method[J].Food and Fermentation Industries,2005,31(11):60-62. |
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| Authors: | Tang Lei Yu Xiaobin Hao Xuecai Hu Weizhen |
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| Affiliation: | School of Biotechnology, The Key Laboratory of Industrial Biotechnology, Ministry of Education,Southern Yangtze University, Wuxi, 214036, China |
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| Abstract: | Optimization of liquid culture medium including maltose, glucose and tryptone for nucleotides production with Cordvceps sinensis CS-wx was carried out by response surface method (RSM). The model to express the content of nucleotide was determined. Regression analysis showed that the contents and the proportion of maltose, glucose and tryptone in the medium influenced the content of nucleotides significantly. According to the ridge analysis, the concentrations of remarkable factors are maltose 2.17％, glucose 2.95％, tryptone 2.74％, the content of adenosine detected by HPLC is 1.02mg/g, improved by 20％ comparing with that in the initial culture medium which content is 0.85 mg/g. |
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| Keywords: | Cordyceps sinensis nucleotides adenosine response surface method ridge analysis HPLC |
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