Actinobacillus succinogenes NJ113厌氧发酵产丁二酸培养条件的优化

采用Plackett-Burman设计法(Plackett-Burman,PB),对影响Actinobacillus succinogenes NJ113厌氧发酵生产丁二酸的11个因子进行了筛选。结果表明,影响该菌厌氧发酵产丁二酸的主要因子为葡萄糖、酵母膏、玉米浆。在此基础上,采用响应曲面法(Response Surface Methodology,RSM)对这3个因子的影响进行了研究,得出丁二酸产量的数学模型,通过对二次多项回归方程求解,得到3因子的最优用量:葡萄糖107g/L,酵母膏16 g/L,玉米浆12 g/L,在优化条件下培养48 h,丁二酸的产量由原始培养条件下的62g/L增到84 g/L,收率从62%提高到78.5%,生产强度达1.75 g/(L·h)。

Optimization of the Culture Conditions for the Production of Succinic Acid by Anaerobic Fermentation of Actinobacillus succinogenes NJ113

Plackett-Burman design was used to evaluate the effects of the eleven factors which influence the production of succinic acid by anaerobic fermentation of Actinobacillus succinogenes NJ113.The signifi- cant ones among the eleven factors were determined as follows:glucose,yeast extract and corn steep liquid (CSL).The response surface methodology(RSM)was used to optimize the three factors.By solving the quadratic regression model equation,the optimal conditions of these variables were determined as:glucose 107g/L,yeast extract 16g/L,CSL 12g/L.After culturing under the optimized condition for 48 hours,the concentration of succinic acid increased from original 62g/L to 84g/L,the yield increased from original 62% to 78.5% with the productivity of 1.75 g/(L·h).