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酶促水解大豆分离蛋白的研究
引用本文:赵新淮 冯志彪. 酶促水解大豆分离蛋白的研究[J]. 食品与发酵工业, 1994, 0(5): 7-11
作者姓名:赵新淮 冯志彪
作者单位:东北农业大学食品科学系
摘    要:本研究利用大豆分离蛋白为原料,通过正交设计试验,确定了碱性蛋白酶的适宜水解条件为:T=55℃,pH=8.0,S=10%,E/S=4%,并得到了收率高、水溶性好的水解蛋白。氨基酸组成表明水解蛋白中除含硫氨基酸外,其它六种必需氨基酸有较好的平衡,与NAS(1980)及FAO(1973)的参考模式相接近。

关 键 词:大豆分离蛋白,蛋白酶,必需氨基酸

Study on the Enzymic Hydrolysis of Soy Isolated Protein
Zhao Xinhuai, Fong Zhibiao,Yu Guopin Zhang Yongzhong, Hao Suer, Zhang Wei. Study on the Enzymic Hydrolysis of Soy Isolated Protein[J]. Food and Fermentation Industries, 1994, 0(5): 7-11
Authors:Zhao Xinhuai   Fong Zhibiao  Yu Guopin Zhang Yongzhong   Hao Suer   Zhang Wei
Abstract:In this paper the soy isolated protein was utilized as raw material and its optimum hydrolysis conditions of a alkaline proteolytic enzyme were determined by orthogonal design test. It was found that T= 55℃,pH8. 0, S= 10% and E/S= 4% are the preferred conditions and the hydrolysed protein thus obtained have a high yield with good solubility. The composition of essential amino acids of hydorlysed protein showed that Ile, Leu, Lys, Phe+Tyr, Thr and Val. have good blance closed to the reference patterns of NAS(1980) and FAO(1973) besides S-containing aminoacids.
Keywords:Soy isolated protein   Proteolytic enzyme   Essential amino acids
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