两步固态法发酵降解山黧豆毒素的研究

从保存的菌种中筛选到B_2和B_(13)2株菌,通过2步固态发酵,在山黧豆培养基中先接菌株B_2培养3d (37℃),灭菌后再接菌株B_(13),培养3d(37℃),灭菌后用0.2 mol/L的HClO_4在0～4℃提取1h,然后利用2,4-二硝基氟苯(FDNB)为柱前衍生试剂,通过高效液相色谱(HPLC),检测了对照和处理中α,β-草酰氨基丙氨酸(ODAP)的含量及降解率。结果发现,对照中β-ODAP和α-ODAP平均含量分别为1.146 mg/g和0.653 mg/g,处理中β-ODAP和α-ODAP的平均含量分别为0.203 mg/g和0.0909 mg/g,处理中的β-ODAP和α-ODAP比对照中的β-ODAP和α-ODAP的平均含量降低了82.2%和86%,而且发酵后处理的蛋白含量明显高于对照。

Reduction Efficiency of the Neurotoxin β-ODAP in Lathyrus sativus by Two Successive Solid State Fermentation

Two strains B_2 and B_(13) degraded from the toxinβ-ODAP were screened and selected.Two suc- cessive solid state fermentations are processed for the two selected strains.After sterilization the seeds were inoculated with B_2.The fermentation was carried out at 37℃for 3 days and was stopped by steam.The sec- ond inoculum B_(13) was added after cooling,and the second fermentation period was processed at 37℃for 3 days and was stopped by steam.The control and processed seed powders were extracted for 1 hour at 0～4℃with 0.2mol/L HClO_4.The contents ofα,β-ODAP were measured by precolumn derivatization with 1-fluo- ro-2,4-dinitrobenzene(FDNB).As a result,the contents ofβ-ODAP andα-ODAP in control seed powders were 1.146mg/g and 0.653 mg/g,respectively.The contents ofβ-ODAP andα-ODAP processed seed pow- ders were 0.203mg/g and 0.0909 mg/g.After fermentation,the detoxification efficiency ofβ-ODAP was 82. 2% and the content ofα-ODAP was decreased by 86%,and the protein level of the processed was significant- ly surpassed that of the control.