| 固定化对动植物酯酶性质的影响 |
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| 引用本文： | 许学勤,徐斐,吴燕雯,吴瑛,华泽钊. 固定化对动植物酯酶性质的影响[J]. 食品与发酵工业, 2004, 30(7): 38-42 |
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| 作者姓名： | 许学勤 徐斐 吴燕雯 吴瑛 华泽钊 |
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| 作者单位： | 上海理工大学食品科学与工程研究所,上海,200093 |
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| 基金项目： | 国家自然科学基金会项目 (No.5 0 1 76 0 32 )，上海市科委“启明星”计划资助项目 (0 2QF1 4 0 30 )，上海市教委青年基金项目(0 2GQ2 1 )资助 |
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| 摘 要： | 比较了离子交换法得到的固定化小麦酯酶和固定化鸡肝酯酶的主要性质与相应的游离酶的性质。结果表明 ,固定化小麦酯酶的最适pH在 5 5附近 ,比游离小麦酶的最适 pH增加了0 8个 pH单位 ;固定化鸡肝酯酶的最适pH在 8 0附近 ,比游离鸡肝酯酶的最适 pH增加了 0 5个pH单位 ;固定化小麦酯酶的最适温度与游离酶的相当 ,但酶活力 温度曲线的峰形变小 ;固定化鸡肝酯酶的最适温度比游离鸡肝酯酶的下降了 5℃ ,酶活力 温度曲线的峰形也略变陡峭。这 2种固定化酯酶对有机磷类农药 (敌敌畏 )抑制的响应明显高于游离酶对农药抑制的响应
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| 关 键 词： | 固定化 小麦酯酶 离子交换树脂 有机磷农药 |
| 修稿时间： | 2004-03-17 |
Effects of Immobilization on Properties of Esterase from Plant and Animal |
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| Xu Xueqin Xu Fei Wu Yanwen Wu Ying Hua Zezhao. Effects of Immobilization on Properties of Esterase from Plant and Animal[J]. Food and Fermentation Industries, 2004, 30(7): 38-42 |
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| Authors: | Xu Xueqin Xu Fei Wu Yanwen Wu Ying Hua Zezhao |
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| Abstract: | Esterases from wheat and chicken liver were immobilized by ion exchanging method The properties of immobilized wheat and chicken liver esterase have been compared with their corresponding free enzymes The results showed that the optimum pH values of both immobilized wheat esterase (IWE) and chicken liver esterase (ICLE) were 0 8 and 0 5 pH unit higher than those of their free esterases respectively, the optimum temperature of IWE was similar to that of free wheat esterase while the optimum temperature of ICLE reduced 5℃ from that of its corresponding free enzyme Both IWE and ICLE became more sensitive to the inhibition of organophosphate pesticide (dichlorvos) than their free enzymes |
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| Keywords: | immobilized wheat esterase chicken liver esterase inhibition |
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