| 肉制品中志贺氏菌DNA的快速提取方法 |
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| 引用本文： | 陈伟,李正国,杨平,杨迎伍,王国民.肉制品中志贺氏菌DNA的快速提取方法[J].食品与发酵工业,2009,35(5). |
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| 作者姓名： | 陈伟 李正国 杨平 杨迎伍 王国民 |
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| 作者单位： | 重庆大学生物工程学院基因工程研究中心,重庆,400030;重庆市高校功能基因与调控新技术重点实验室,重庆,400030 |
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| 基金项目： | 国家自然科学基金，重庆市科委自然科学基金重点项目 |
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| 摘 要： | 肉制品中致病菌DNA的提取对PCR检测具有重要意义。以污染志贺氏菌的猪肉为材料,比较了沸水浴法、裂解液煮沸法、CTAB/SDS法、改良的碱性异硫氰酸胍法4种提取方法对志贺氏菌DNA的提取效果,并以提取的DNA为模板进行PCR检测。结果表明:改良的碱性异硫氰酸胍法提取志贺氏菌DNA的效果较好,猪肉中志贺氏菌的PCR最低检出限为5×100CFU/g,整个检测时间<8h。经改良的碱性异硫氰酸胍法操作简便、经济省时,可用于肉类的PCR检测。
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| 关 键 词： | 肉制品 志贺氏菌 DNA 提取 PCR |
The Study on Rapid DNA Extraction Method of Shigella from Meat Products |
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| Chen Wei,Li Zhengguo,Yang Ping,Yang Yingwu,Wang Guoming.The Study on Rapid DNA Extraction Method of Shigella from Meat Products[J].Food and Fermentation Industries,2009,35(5). |
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| Authors: | Chen Wei Li Zhengguo Yang Ping Yang Yingwu Wang Guoming |
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| Abstract: | The DNA extraction of pathogenic bacteria from meat products is very important for PCR detection.Four DNA extraction methods including boiling method,lysis method,CTAB/SDS method and modified alkaline guanidine thiocyanate method were used for Shigella DNA extraction from the contaminated pork with Shigella,and the extracted DNA was used as a template for PCR detection.The results showed that the modified alkaline guanidine thiocyanate method was better than others,the sensitivity of PCR detection was 5×10 0 CFU/g in pork and the detection procedure was less than 8 hours.This DNA extraction method is simple,rapid and cheap;it is suitable for PCR detection of meat in practical application. |
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| Keywords: | DNA PCR |
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