| 大孔树脂富集纯化胡芦巴种子总黄酮 |
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| 引用本文： | 杨仁明,何彦峰,索有瑞,王洪伦.大孔树脂富集纯化胡芦巴种子总黄酮[J].食品与发酵工业,2012(2):224-228. |
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| 作者姓名： | 杨仁明 何彦峰 索有瑞 王洪伦 |
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| 作者单位： | 中国科学院西北高原生物研究所;中国科学院研究生院 |
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| 基金项目： | 中国科学院生命科学领域优秀青年科技专项(KSCX2-EW-Q-23) |
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| 摘 要： | 通过比较11种大孔吸附树脂对胡芦巴黄酮类化合物的静态吸附与解吸性能,筛选出DM130型大孔吸附树脂用于分离纯化胡芦巴种子中的黄酮类化合物。采用单因素方法分析该树脂富集纯化胡芦巴总黄酮的适宜工艺条件,确定优化的工艺条件为:上样量为3.64 mg黄酮/g树脂,上样液pH值5.0,吸附时间2 h,体积分数70%乙醇洗脱,洗脱速率2 mL/min,洗脱体积为150 mL,总黄酮回收率为85.05%,提取物中黄酮含量由7.8%提高到26.5%。
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| 关 键 词： | 胡芦巴 大孔吸附树脂 总黄酮 富集纯化 |
Purification of Flavonoids from Trigonella foenum-graecum L.Seeds by Macroporous Adsorption Resin |
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| Yang Ren-ming,He Yan-feng,Suo You-rui,Wang Hong-lun.Purification of Flavonoids from Trigonella foenum-graecum L.Seeds by Macroporous Adsorption Resin[J].Food and Fermentation Industries,2012(2):224-228. |
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| Authors: | Yang Ren-ming He Yan-feng Suo You-rui Wang Hong-lun |
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| Affiliation: | 1(Northwest Institute of Plateau Biology,Chinese Academy of Sciences,Xining 810001,China) 2(Graduate university of the Chinese Academy of Sciences,Beijing 100049,China) |
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| Abstract: | Eleven types of macroporous resins were applied to study static adsorption and desorption performances towards Trigonella foenum-graecum L.seeds flavonoids,and DM130 was the selected for the separation and purification of the crude flavonoids from Trigonella foenum-graecum L.prepared with ultrasonic-assisted extraction.Single factor experiments showed that the optimum purification procedure of total flavonoids from Trigonella foenum-graecum L.were determined as follows: sample loading amount 3.64 mg total flavonoids /g macroporous adsorption resin at pH 5.0,adsorption duration 2 h,flow rate 2.0 mL/min,70% ethanol,desorption volume 3BV.Under the above optimized purification process,the recovery of flavonoids was 85.05% and the purity increased from 7.8% to 26.5%. |
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| Keywords: | Trigonella foenum-graecum L macroporous adsorption resin total flavonoids enrichment and purification |
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