副干酪乳杆菌LPC-F通过促进Cajal间质细胞增殖缓解便秘

便秘(constipation)是一类常见的胃肠道疾病,其发病率呈逐年上升的趋势。临床研究发现,便秘患者肠道菌群失调。通过赋予机体一定量的活性益生菌来调节肠道菌群,已然成为治疗便秘的重要手段。因此,该研究选取2株具有不同缓解便秘效果的副干酪乳杆菌进行研究。通过对便秘相关指标的检测,再次证实仅有来源于健康成人粪便中的副干酪乳杆菌LPC-F具有缓解便秘的作用。进一步通过对便秘相关胃肠调节肽、血清中炎症因子、结肠Cajal间质细胞的数量、肠道菌群及其代谢产物短链脂肪酸等指标进行检测,解析副干酪乳杆菌LPC-F缓解便秘的可能方式。结果表明副干酪乳杆菌LPC-F可能通过改变肠道菌群的结构,提高肠道内乙酸的水平,乙酸则通过刺激肠嗜铬细胞释放5-羟色胺(5-hydroxytryptamine, 5-HT),释放入肠腔的5-HT通过与其受体相结合,促进结肠Cajal间质细胞(interstitial cells of Cajal, ICC)的增殖,从而最终增强肠蠕动缓解便秘。

Lactobacillus paracasei LPC-F relieved constipation by promoting the proliferation of interstitial cells of Cajal

Constipation is a common gastrointestinal disease,and the incidence is increasing year by year.Clinical studies have found that the intestinal microbiota of patients with constipation is imbalanced.Regulating the intestinal microbiota by giving the host a certain amount of active probiotics has become an important means of treating constipation.In this study,two strains of Lactobacillus paracasei with different constipation relief effects were selected for research.It was once again confirmed that only L.paracasei LPC-F derived from the feces of healthy adults had the effect of relieving constipation by detecting the constipation-related indicators.Furthermore,we analyzed the possible ways of L.paracasei LPC-F to relieve constipation by detecting the constipation-related gastrointestinal regulatory peptides,serum inflammatory factors,the number of colonic Cajal interstitial cells,intestinal microbiota and its metabolite SCFAs and other indicators.The results indicated that L.paracasei LPC-F increased the level of acetic acid by changing the structure of the intestinal microbiota.Acetic acid stimulated the release of 5-HT from enterochromaffin cells,and 5-HT,which released into the intestinal lumen,bound to its receptor to promote the proliferation of interstitial cells of Cajal,thereby ultimately enhancing intestinal peristalsis and alleviating constipation.