| 嗜热拟青霉利用玉米芯产木聚糖酶的发酵条件优化 |
| |
| 引用本文： | 田红梅,江正强,杨绍青,李里特.嗜热拟青霉利用玉米芯产木聚糖酶的发酵条件优化[J].食品与发酵工业,2006,32(5):1-4. |
| |
| 作者姓名： | 田红梅 江正强 杨绍青 李里特 |
| |
| 作者单位： | 中国农业大学食品科学与营养工程学院,北京,100083 |
| |
| 基金项目： | 全国高等学校优秀博士学位论文作者专项基金;高比容电子铝箔的研究开发与应用项目 |
| |
| 摘 要： | 以一株新的嗜热拟青霉(Paecilomyces thermophila)J18为出发菌株,对其液态发酵产木聚糖酶的条件进行了优化。结果表明,以玉米芯为碳源能高效诱导木聚糖酶的胞外分泌,胰蛋白胨为最佳氮源,初始pH和培养温度分别为pH 7.5,50℃,所得酶活力最高。在优化后的培养基和培养条件下,第5 d酶活力达到峰值,为1 276 U/mL,表明该拟青霉能够利用农业废弃物高效生产木聚糖酶。该酶水解桦木木聚糖主要生成木二糖和木三糖,适合生产低聚木糖。
|
| 关 键 词： | 发酵条件 木聚糖酶 嗜热拟青霉 玉米芯 |
| 收稿时间： | 12 14 2005 12:00AM |
| 修稿时间： | 03 3 2006 12:00AM |
Utilization of Corncob for Xylanase Production by Paecilomyces thermophila |
| |
| Tian Hongmei,Jiang Zhengqiang,Yang Shaoqing,Li Lite.Utilization of Corncob for Xylanase Production by Paecilomyces thermophila[J].Food and Fermentation Industries,2006,32(5):1-4. |
| |
| Authors: | Tian Hongmei Jiang Zhengqiang Yang Shaoqing Li Lite |
| |
| Affiliation: | College of Food Science and Nutritional Engineering, China Agriculture University, Beijing 100083 ,China |
| |
| Abstract: | Fermentation conditions of a newly isolated Paecilomyces thermophila J18 for xylanase production were investigated in this study.Corncob was found to be the best inducer and carbon source,while tryptone was the best nitrogen source for the xylanase production.The optimal initial pH and temperature of the cultures were pH 7.5 and 50℃,respectively.Highest xylanase activity of 1 276 U/mL was achieved under the optimal condition on the fifth day of incubation,showing the feasibility of xylanase production using corncob as carbon source. The major products of birchwood xylan hydrolyzed by the xylanase were xylobiose and xylotriose,suggesting the xylanase is suitable for xylooligosaccharides production. |
| |
| Keywords: | fermentation conditions xylanase Paecilomyces thermophila corncob |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
