| 灰绿曲霉突变株降解稻草秸秆及联合产氢的研究 |
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| 引用本文： | 杜娟,林觐勤,徐昶,邬小兵,徐惠娟,龙敏南.灰绿曲霉突变株降解稻草秸秆及联合产氢的研究[J].食品与发酵工业,2007,33(2):18-21. |
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| 作者姓名： | 杜娟 林觐勤 徐昶 邬小兵 徐惠娟 龙敏南 |
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| 作者单位： | 厦门大学生命科学学院,厦门大学生物能源中心,福建厦门,361005 |
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| 基金项目： | 国家高技术研究发展计划(863计划);福建省科技计划;福建省厦门市科技计划项目 |
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| 摘 要： | 对灰绿曲霉(Aspergillus glaucus)突变株EU7-22进行单因子和正交实验,确定菌株利用稻草产纤维素酶的液态发酵优化组合条件为:稻草粉3%、蛋白胨0.5%、温度35℃、起始pH 5。在优化条件下培养4~5 d可达到产酶高峰。利用优化培养后的酶液水解稻草粉产糖,在最适条件下,稻草粉酶解反应24 h后,糖化率可达49%。利用稻草粉做底物,EU7-22和产氢克雷伯氏菌HP1联合发酵,1 g稻草粉可产氢23.7 mL。
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| 关 键 词： | 灰绿曲霉 突变株 纤维素酶 秸秆糖化 生物制氢 |
| 修稿时间： | 07 5 2006 12:00AM |
Degradation of Rice Straw by Aspergillus glaucus Mutant and integration with Biohydrogen Production |
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| Du Juan,Lin Jinqin,Xu Chang,Wu Xiaobing,Xu Huijuan,Long Minnan.Degradation of Rice Straw by Aspergillus glaucus Mutant and integration with Biohydrogen Production[J].Food and Fermentation Industries,2007,33(2):18-21. |
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| Authors: | Du Juan Lin Jinqin Xu Chang Wu Xiaobing Xu Huijuan Long Minnan |
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| Affiliation: | School of Life Sciences, Bio-Energy Center, Xiamen University, Xiamen 361005, China |
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| Abstract: | The optimal conditions for cellulase production of Aspergillus glaucus mutant strain EU7-22 was achieved as follows: rice straw powder quantity 3%,peptone quantity 0.5%,original pH 5,and temperature 35℃,by orthogonal experiments.The strain reached its maximum cellulose activity after 4~5 days fermentation.When the cellulase was applied for saccharification of rice straw,a saccharification rate of 49% was obtained after 24h hydrolysis.The saccharification liquid was subsequently used for hydrogen production.A yield of 23.7 mL H2/g rice powder was acquired when using Klebsiella oxytoca HP1 as the hydrogen producer. |
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| Keywords: | Asperillus glaucus mutant strain cellulase straw saccharification biohydrogen production |
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