| 采用响应面法优化木糖醇发酵培养基 |
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| 引用本文： | 邓立红,张扬,马润宇,王艳辉.采用响应面法优化木糖醇发酵培养基[J].食品与发酵工业,2005,31(6):59-61. |
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| 作者姓名： | 邓立红 张扬 马润宇 王艳辉 |
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| 作者单位： | 北京化工大学生命科学院制药工程系,北京,100029 |
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| 摘 要： | 将Plackett-Burman和响应面设计相结合,对木糖醇发酵培养基进行了优化。结果表明,初始木糖浓度、酵母膏添加量以及MgSO4.7H2O浓度是影响木糖醇转化率的主要因素。优化得到的培养基组成为(g/L)木糖100.7,酵母膏5.302,NaCl6.0,MgSO4.7H2O0.379,KH2PO43,(NH4)2HPO44;通气条件为装液量100mL/250mL。此条件下木糖醇的转化率为0.784g/g。
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| 关 键 词： | 热带假丝酵母 Plackett-Burman 响应面法 |
| 修稿时间： | 2004年11月12 |
Optimizing Xylitoi Fermentation Meadium by Method of Response Surface Analysis |
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| Deng Lihong,Zhang Yang,MA Runyu,Wang Yanhui.Optimizing Xylitoi Fermentation Meadium by Method of Response Surface Analysis[J].Food and Fermentation Industries,2005,31(6):59-61. |
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| Authors: | Deng Lihong Zhang Yang MA Runyu Wang Yanhui |
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| Abstract: | Plackett-burman and response surface analysis(RSA) methods were applied to optimize fermentation meadium of xylitol fermentation. The results indicated that xylitol yield was mainly influenced by concentration of initial xylose,yeast extract and magnesium sulphate.The obtained optimal fermentation meadium components(g/L)were xylose 100.7, yeast extract 5.302, MgSO_4·7H_2O 0.379, NaCl 6.0,KH_2PO_4 3,(NH_4)_2HPO_4 4,and culture meadium volume of 100 mL/250 mL was the optimized aeration condition. Under these conditions,the maximum xylitol yield in theory was 0.779 g/g,and the practical value was 0.784 g/g. |
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| Keywords: | Candida tropicalis Plackett-Burman response surface analysis |
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