| 南极磷虾中不同形态虾青素的分离制备、结构鉴定及含量分析 |
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| 引用本文： | 丛心缘,孙伟红,张辉珍,冷凯良,邢丽红,苗钧魁,刘小芳.南极磷虾中不同形态虾青素的分离制备、结构鉴定及含量分析[J].食品与发酵工业,2019(2):174-178. |
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| 作者姓名： | 丛心缘 孙伟红 张辉珍 冷凯良 邢丽红 苗钧魁 刘小芳 |
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| 作者单位： | 青岛大学公共卫生学院;中国水产科学研究院黄海水产研究所农业部极地渔业开发重点实验室;海洋国家实验室海洋药物与生物制品功能实验室;青岛市食品药品监督管理局 |
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| 基金项目： | 中国水产科学研究院基本科研业务费专项(2016HY-ZD0902)资助 |
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| 摘 要： | 以南极磷虾为研究对象,采用丙酮提取南极磷虾中的虾青素。优化硅胶柱层析技术分离虾青素双酯、单酯及游离虾青素;筛选合适的展开剂,利用薄层层析法对得到的样品进行鉴定;通过Chiralpak IC柱鉴别其立体结构;运用C30-HPLC法测定不同形态虾青素的含量。结果表明,硅胶柱层析以不同比例的丙酮-石油醚溶液梯度淋洗,可以分离制备虾青素双酯、单酯及游离虾青素; V(正己烷)∶V(丙酮)∶V(乙酸)=8∶2∶0. 2为薄层层析最佳展开剂。南极磷虾中不同形态虾青素均由3S,3'S、3S,3'R、3R,3'R 3种光学异构体组成,虾青素双酯和单酯中3R,3'R所占比例> 70%,3S,3'S的比例最低。南极磷虾中总虾青素含量为18. 9 mg/kg,其中虾青素双酯中虾青素含量为12. 0 mg/kg,虾青素单酯中虾青素含量为6. 1 mg/kg,游离虾青素含量为0. 8 mg/kg。
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| 关 键 词： | 南极磷虾 虾青素酯 硅胶柱层析 光学异构体 |
Separation,structure identification and content analysis of different forms of astaxanthin in Antarctic krill |
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| CONG Xinyuan,SUN Weihong,ZHANG Huizhen,LENG Kailiang,XING Lihong,MIAO Junkui,LIU Xiaofang.Separation,structure identification and content analysis of different forms of astaxanthin in Antarctic krill[J].Food and Fermentation Industries,2019(2):174-178. |
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| Authors: | CONG Xinyuan SUN Weihong ZHANG Huizhen LENG Kailiang XING Lihong MIAO Junkui LIU Xiaofang |
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| Affiliation: | (Qingdao University,School of Public Health,Qingdao 266071,China;Yellow Sea Fisheries Research Institute,ChineseAcademy of Fishery Sciences,Key Laboratory of Sustainable Development of Polar Fishery,Ministry of Agriculture,Qingdao266071,China;Qingdao Food and Drug Administration,Qingdao 266071,China;Laboratory for Marine Drugs andBioproducts of Qingdao National Laboratory for Marine Science and Technology,Qingdao 266071,China) |
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| Abstract: | CONG Xinyuan;SUN Weihong;ZHANG Huizhen;LENG Kailiang;XING Lihong;MIAO Junkui;LIU Xiaofang(Qingdao University,School of Public Health,Qingdao 266071,China;Yellow Sea Fisheries Research Institute,ChineseAcademy of Fishery Sciences,Key Laboratory of Sustainable Development of Polar Fishery,Ministry of Agriculture,Qingdao266071,China;Qingdao Food and Drug Administration,Qingdao 266071,China;Laboratory for Marine Drugs andBioproducts of Qingdao National Laboratory for Marine Science and Technology,Qingdao 266071,China) |
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| Keywords: | Antarctic krill astaxanthin ester silica gel column chromatography optical isomers |
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