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聚酰胺吸附-硝酸铝显色法测定山楂及山楂制品中的总黄酮含量
引用本文:元晓梅,蒋明蔚.聚酰胺吸附-硝酸铝显色法测定山楂及山楂制品中的总黄酮含量[J].食品与发酵工业,1996(4):27-32.
作者姓名:元晓梅  蒋明蔚
摘    要:本文介绍了经系统研究确定的测定山楂及山楂制品中总黄酮的比色法。样品经乙醇抽提,聚酰胺吸附提纯,以Al(NO_3)_3显色法于510nm下测定,以芦丁为标准计算含量,该方法最低检出浓度为0.12%,相对标准偏差为0.028~0.24%,平均回收率为102±7.1%,测定结果与其它方法无显著性差异。

关 键 词:总黄酮,山楂,比色法

Colorimetric Determination of Flavones in Hawthorn and Its Products
Abstract:colorimetric method for the determination of flavones in hawthron through systematic research was described. Extracted by ethanol and purified by polycaprolactam, flavones produced colour with aluminium nitrate that was quantitated by measurement of the absorbance at 510nm. Tlie rutin was used as a standard compound.The lowest limit of determination was 0. 12%.The precision(RSD%)was 0. 028~0.24.The recovery was 1 02. 0±7. 1%. Determination results showed satisfactory agreement with the other method.
Keywords:Flavones  Hawthron  Colorimetric method
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