| EGCG甲基化衍生物的清除DPPH自由基活性和总抗氧化活性 |
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| 引用本文： | 吕海鹏,林智,谭俊峰,郭丽,张悦,费旭元.EGCG甲基化衍生物的清除DPPH自由基活性和总抗氧化活性[J].食品与发酵工业,2011,37(9). |
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| 作者姓名： | 吕海鹏 林智 谭俊峰 郭丽 张悦 费旭元 |
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| 作者单位： | 国家茶产业工程技术研究中心,农业部茶及饮料植物产品加工与质量控制重点开放实验室,中国农业科学院茶叶研究所,浙江杭州310008 |
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| 基金项目： | 浙江省自然科学基金项目(Y307498和Y3080088); 现代农业产业技术体系建设专项(nycytx-26) |
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| 摘 要： | 采用制备色谱技术,通过甲基化技术得到的甲基化儿茶素粗品中,分离纯化出3个甲基化表没食子儿茶素没食子酸酯(EGCG)的单体,结合LC/MS和NMR等分别确定为4',4″-di-Me-EGCG、4',3″,4″-tri-Me-EGCG以及4',3″,4″,5″-tetra-Me-EGCG;进一步比较了这3个EGCG甲基衍生物单体与98%EGCG在清除DPPH自由基活性和总抗氧化活性等方面的差异。结果表明:EGCG甲基化后清除DPPH自由基活性和总抗氧化活性都有明显的降低,总体上分别只有98%EGCG的68.17%和6.84%。
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| 关 键 词： | EGCG 甲基化衍生物 总抗氧化活性 |
DPPH Scavenging Activity and Total Antioxidant Activity of Methylated EGCG Derivatives |
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| Lv Hai-peng,Lin Zhi,Tan Jun-feng,Guo Li,Zhang Yue,Fei Xu-yuan.DPPH Scavenging Activity and Total Antioxidant Activity of Methylated EGCG Derivatives[J].Food and Fermentation Industries,2011,37(9). |
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| Authors: | Lv Hai-peng Lin Zhi Tan Jun-feng Guo Li Zhang Yue Fei Xu-yuan |
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| Affiliation: | Lv Hai-peng,Lin Zhi,Tan Jun-feng,Guo Li,Zhang Yue,Fei Xu-yuan(National Engineering Research Center for Tea Processing,Key Laboratory of Processing and Quality Control of Tea and Beverage Plants,Ministry of Agriculture,Tea Research Institute,Chinese Academy of Agricultural Sciences,Hangzhou 310008,China) |
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| Abstract: | Three methylated EGCG derivatives were purified by preparative chromatography,and their structures were identified by LC/MS and NMR as the following: 4′,4″-di-Me-EGCG、4′,3″,4″-tri-Me-EGCG and 4′,3″,4″,5″-tetra-Me-EGCG.DPPH scavenging assay and the evaluation of total antioxidant activity experiment showed that the methylated EGCG derivatives had much lower antioxidant capability than 98% EGCG,only to 68.17% and 6.84% of 98% EGCG in average,respectively. |
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| Keywords: | EGCG methylate derivatives total antioxidant activity |
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