鲍鱼脏器糖蛋白超声辅助提取工艺及分离纯化的研究

以冻藏鲍鱼脏器为试验原料,分析了鲍鱼脏器的基本成分组成,研究了鲍鱼脏器糖蛋白的分离纯化及纯化物基本组成。通过单因素试验、正交试验确定了最适提取条件为:5%NaCl溶液、料液质量比1∶9、超声功率为300 W、超声处理时间为40 min,在此条件下,糖蛋白提取率为69.7 mg/g(干重),比传统方法提高了28.5%。糖蛋白粗品经硫酸铵沉淀,DEAE-Cellulous 52柱层析,SephacrylS-300 HR柱层析后,经SDS-PAGE电泳鉴定得到分子质量为73.7 ku的组成均一的糖蛋白AGP-Ⅱ,AGP-Ⅱ含有总糖12.4%,总蛋白76.6%。

Study on the Ultrasound-assisted Extraction and Purification of Abalone Visceral Glycoprotein

Frozen abalone visceral were used as materials.The general components of abalone visceral were analyzed.The optimized conditions of ultrasound-assisted glycoprotein extraction was selected by orthogonal analysis on the basis of single factor experiments.The optimized conditions of ultrasound-assisted extraction were as follows: NaCl concentration was 5%,the ratio of material to solvent was 1∶ 9,ultrasonic power was 300 W and ultrasonic time was 40 min.The extraction rate of glycoprotein in the extraction was 69.7mg/g by this method,the extraction ratio increased by 28.5% compared to the tradition method.Furthermore,the glycoproteins were precipitated by(NH4)2SO4 and isolated by DEAE-Cellulose and SephacrylS-300 HR.Results showed that a homogeneous glycoprotein(AGP-Ⅱ) was obtained.The molecular weight of AGP-Ⅱ was estimated to be 73.7 ku by SDS-PAGE.AGP-Ⅱ contained 12.4% total sugar,76.6% protein.