| 发状念珠藻藻蓝蛋白分离纯化及性质研究 |
| |
| 引用本文： | 岳思君,贾士儒,丁文杰,戴玉杰. 发状念珠藻藻蓝蛋白分离纯化及性质研究[J]. 食品与发酵工业, 2011, 37(9) |
| |
| 作者姓名： | 岳思君 贾士儒 丁文杰 戴玉杰 |
| |
| 作者单位： | 1. 天津科技大学,工业微生物教育部重点实验室,天津300457／宁夏大学生命科学学院,宁夏银川750021
2. 天津科技大学,工业微生物教育部重点实验室,天津300457 |
| |
| 基金项目： | 国家自然科学基金(20776112,20376061); 国家“十一五”支撑计划项目(2006BAD27B06) |
| |
| 摘 要： | 采用冻融法破碎发状念珠藻细胞,经过磷酸缓冲液提取、硫酸铵分步沉淀、离子交换层析和凝胶层析分离纯化发状念珠藻藻蓝蛋白,其纯度(A615/A280)达到5.321,纯化因子为7.623。纯化的藻蓝蛋白最大吸收峰为615 nm,其室温荧光发射峰为646 nm,SDS-PAGE电泳显示其α和β亚基的分子质量在14.4～18.4 ku。稳定性实验表明,低温、避光、pH 6～8以及低浓度的蔗糖等条件下发状念珠藻藻蓝蛋白是稳定的。实验结果将为开发利用发状念珠藻藻蓝蛋白提供参考。
|
| 关 键 词： | 发状念珠藻 藻蓝蛋白 离子交换层析 凝胶层析 |
Purification and Characterization of C-phycocyanin from Liquid Cultured Nostoc flagelliforme Cells |
| |
| Yue Si-jun,,Jia Shi-ru,Ding Wen-jie,Dai Yu-jie. Purification and Characterization of C-phycocyanin from Liquid Cultured Nostoc flagelliforme Cells[J]. Food and Fermentation Industries, 2011, 37(9) |
| |
| Authors: | Yue Si-jun Jia Shi-ru Ding Wen-jie Dai Yu-jie |
| |
| Affiliation: | Yue Si-jun1,2,Jia Shi-ru1,Ding Wen-jie1,Dai Yu-jie1 1(Key Laboratory of Industrial Microbiology,Ministry of Education,Tianjin University of Science and Technology,Tianjin 300457,China) 2(School of Life Science,Ningxia University,Yinchuan 750021,China) |
| |
| Abstract: | It's a useful measure for the protection of the endangered natural resources to extract useful substances from artificial cultured species.In this study,C-phycocyanin was extracted from liquid-cultured Nostoc flagelliforme cells with repeated frost thawing followed by frozen ultrasonic method.The crude extract was successfully purified by procedure of ammonium sulfate precipitation,ion exchange chromatography with DEAE 650M column,and gel filtration chromatography with Sephadex G200 column.The purity ratio ... |
| |
| Keywords: | Nostoc flagelliforme C-phycocyanin ion exchange chromatography gel filtration chromatography |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
