氮源对2株海洋微藻生长及脂肪酸合成的影响

以三角褐指藻、扁藻为材料,研究了NaNO3、尿素和NH4Cl等不同氮源对其生长、叶绿素、可溶性糖、可溶性蛋白、干重、总脂及脂肪酸含量的影响。结果表明:以尿素为氮源时,三角褐指藻的生物量、生长速率、叶绿素、可溶性糖、可溶性蛋白的含量最高,NaNO3和NH4Cl次之;以NH4Cl为氮源时,细胞内的总脂含量最高,占干重的47.5℅。与三角褐指藻不同,以NH4Cl为氮源时,扁藻的生物量、生长速率、叶绿素、可溶性糖、可溶性蛋白的含量最高,尿素和NaNO3次之;当以NaNO3为氮源时,细胞内的总脂含量最高,占干重的38.5℅。

Effects of Different Format Nitrogen on Growth and Fatty Acid Synthesis of Marine Oleaginous Microalgaes

The effects of nitrogen in different format on the growth,biochemical composition,fatty acid composition and content of Phaeodactylum tricornutum and Tetraselmis Chui was investigated.The results showed that: with urea as nitrogen source,the biomass,growth rate,chlorophyll content,soluble sugar and soluble protein contents of Phaeodactylum tricornutum were the highest,followed by sodium nitrate and ammonium chloride as nitrogen source.However,with ammonium chloride as nitrogen source,total lipid content was the highest,and types of fatty acids were the most.Tetraselmis Chui with ammonium chloride as nitrogen source,the biomass,growth rate,chlorophyll content,soluble sugar and soluble protein contents were the highest,followed by urea,sodium nitrate.However,the total lipid content and fatty acid species reached their maximum with sodium nitrate as nitrogen source.