| 糖蜜原料培养高核酸酵母NY-1培养条件优化 |
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| 引用本文： | 王媛媛,曹春红,杨旭,肖冬光.糖蜜原料培养高核酸酵母NY-1培养条件优化[J].食品与发酵工业,2012,38(1):62-65. |
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| 作者姓名： | 王媛媛 曹春红 杨旭 肖冬光 |
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| 作者单位： | 天津科技大学生物工程学院 工业发酵微生物教育部重点实验室,天津,300457 |
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| 摘 要： | 对以糖蜜为原料培养高核酸酵母NY-1的发酵培养条件进行了优化。由实验得知,培养基最适初始pH为5.0,采用酵母浸粉和尿素的复合氮源,菌体生长较好;提高糖蜜中蔗糖水解率,可以延长酵母生长的对数期3h,有利于提高核酸酵母的浓度;采用挡板瓶提高溶氧后培养该酵母,菌体干重可达到15.4 g/L。
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| 关 键 词： | 高核酸酵母 RNA 蔗糖 糖蜜 |
Optimization on Submerged Fermentation Conditions of High Nuclear Saccharomyces NY-1 by Sugar Molasses |
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| Wang Yuan-yuan,Cao Chun-hong,Yang Xu,Xiao Dong-guang.Optimization on Submerged Fermentation Conditions of High Nuclear Saccharomyces NY-1 by Sugar Molasses[J].Food and Fermentation Industries,2012,38(1):62-65. |
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| Authors: | Wang Yuan-yuan Cao Chun-hong Yang Xu Xiao Dong-guang |
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| Affiliation: | (Key Laboratory of Industrial Fermentation Microbiology,Ministry of Education,College of Biotechnology,Tianjin University of Science and Technology,Tianjin 300457,China) |
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| Abstract: | Sugar molasses was used to culture the High nuclear Saccharomyces.The fermentation condition was studied.The optimum medium ingredients and culture condition were obtained as followed:the initial pH 5.0,the complex nitrogen sources of yeast powder and urea;by increasing the rate of the hydrolyzation of the sucrose,the logarithm period of NY-1 is prolonged and the cell density was also increased.The cell dry weight was up to 15.4g/L when the yeast was cultured in baffled flasks. |
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| Keywords: | high nuclear Saccharomyces RNA sucrose sugar molasses |
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