| 叶黄素的分离纯化及其清除羟自由基活性 |
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| 引用本文： | 彭胜,彭密军,黄美娥,张昌伟,张琳杰.叶黄素的分离纯化及其清除羟自由基活性[J].食品与发酵工业,2012,38(3):145-148. |
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| 作者姓名： | 彭胜 彭密军 黄美娥 张昌伟 张琳杰 |
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| 作者单位： | 吉首大学林产化工工程湖南省重点实验室,湖南张家界,427000 |
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| 基金项目： | 湖南省教育厅重点项目，湖南省自然科学基金项目，湖南省高校“林产资源化学与林化产品开发”科技创新团队支持计划项目 |
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| 摘 要： | 运用KOH-甲醇溶液皂化法将叶黄素酯转化为游离叶黄素,进一步采用硅胶柱层析进行分离纯化,并对不同纯度产品中叶黄素的含量进行HPLC法测定;以没食子酸丙酯为参照,采用流动注射化学发光法测定不同纯度叶黄素产品清除.OH的效果。结果表明:叶黄素浸膏通过皂化,游离叶黄素的含量由1.35%提高到30.25%,进一步柱层析处理得到80.32%的叶黄素产品;不同纯度叶黄素产品均具有较强的清除.OH活性,且其抗氧化活性与叶黄素的含量正相关。
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| 关 键 词： | 叶黄素 分离纯化 羟自由基 流动注射化学发光法 |
Separation and Purification of Lutein and Its Scavenging Activities on Hydroxyl Radical |
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| Peng Sheng,Peng Mi-jun,Huang Mei-e,Zhang Chang-wei,Zhang Lin-jie.Separation and Purification of Lutein and Its Scavenging Activities on Hydroxyl Radical[J].Food and Fermentation Industries,2012,38(3):145-148. |
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| Authors: | Peng Sheng Peng Mi-jun Huang Mei-e Zhang Chang-wei Zhang Lin-jie |
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| Affiliation: | (Key Laboratory of Hunan Forest Products and Chemical Industry Engineering,Jishou University,Zhangjiajie 427000,China) |
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| Abstract: | In this paper,the luteinesters was saponated and purified by KOH-methanol methods and silica gel column chromatography,and the content was determined by HPLC.Propyl gallate was used as a positive control,the products of lutein were assessed for their antioxidative effects on hydroxyl.The results demonstrated that the content of lutein was successively increased from 1.35% to 30.25% and 80.32% by saponification and purification.The products of lutein showed obvious scavenging ability to hydroxyl radical,and its antioxidant activity was positively correlated with the content. |
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| Keywords: | lutein separation and purification hydroxyl radical flow Injection chemiluminescence |
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