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| 酱油二次沉淀制备方法的对比 | |
| 引用本文： | 高献礼,孙鹏飞,闫爽,陈燕斌,陆健.酱油二次沉淀制备方法的对比[J].食品与发酵工业,2013,39(1):46-50. |
| 作者姓名： | 高献礼 孙鹏飞 闫爽 陈燕斌 陆健 |
| 作者单位： | 1. 江南大学粮食发酵工艺与技术国家工程实验室,江苏无锡,214122 2. 江南大学工业生物技术教育部重点实验室,江苏无锡,2141222 3. 江南大学生物工程学院,江苏无锡,214122 |
| 基金项目： | 江南大学2011年校自主科研计划项目，江南大学工业生物技术教育部重点实验室开放课题，江苏高校优势学科建设工程资助项目 |
| 摘 要： | 通过静置法、离心法、低温法、超声法及低温-超声法制备了酱油二次沉淀样品,对比研究了其溶解性能、常规指标和氨基酸组成,通过聚类分析法对5种样品的氨基酸组成进行了分析。实验结果表明:5种方法制备的酱油二次沉淀中的蛋白质均不溶解于酸性缓冲溶液(pH<7.0),低温-超声法制备样品的蛋白质含量明显高于其他4种方法制备样品的蛋白质含量,且其相应的氨基酸组成也与其他4种方法制备样品的氨基酸组成存在明显差异。以上结果说明采用低温-超声法制备酱油二次沉淀样品更为科学和快速。 |
| 关 键 词： | 酱油 二次沉淀 氨基酸组成 聚类分析 |
A comparative study on the different methods for secondary precipitates preparation of soy sauce |
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| Gao Xian-li,Sun Peng-fei,Yan Shuang,Chen Yan-bin,Lu Jian.A comparative study on the different methods for secondary precipitates preparation of soy sauce[J].Food and Fermentation Industries,2013,39(1):46-50. | |
| Authors: | Gao Xian-li Sun Peng-fei Yan Shuang Chen Yan-bin Lu Jian |
| Affiliation: | 1(National Engineering Laboratory for Cereal Fermentation Technology,Jiangnan University,Wuxi 214122,China) 2(Key Laboratory of Industrial Biotechnology,Ministry of Education,Jiangnan University,Wuxi 214122,China) 3(School of Biotechnology,Jiangnan University,Wuxi 214122,China) |
| Abstract: | In this paper,the secondary precipitates of soy sauce were prepared by natural precipitation,centrifugation,cooling,ultrasonic,and cooling-ultrasonic coupling methods.Their solubilities,proximate indices and amino acids composition were studied comparatively.Meanwhile,hierarchical cluster analysis was conducted to analyze the differences of amino acids composition in the above secondary precipitates.Results showed that proteins in the above precipitates could not be re-dissolved in acidic buffers(pH<7.0).The content of protein in precipitate prepared by cooling-ultrasonic coupling method was higher than that in precipitates prepared by other methods.Meanwhile,its amino acids composition was obviously different from those of the other precipitates.The cooling-ultrasonic coupling method was more scientific and quick for secondary precipitate preparation. |
| Keywords: | soy sauce secondary precipitate amino acids composition hierarchical cluster analysis |
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